Recombinant VP28 Produced by Cell-Free Technique Confers Protection in Kuruma Shrimp (Marsupenaeus japonicus) Against White Spot Syndrome Virus

被引：9

作者：

Kono, T. ^{[1
]}

Fall, J. ^{[2
]}

Korenaga, H. ^{[3
]}

Sudhakaran, R. ^{[4
]}

Biswas, G. ^{[5
]}

Mekata, T. ^{[6
]}

Itami, T. ^{[1
]}

Sakai, M. ^{[1
]}

机构：

[1] Miyazaki Univ, Fac Agr, Dept Biochem & Appl Biosci, Miyazaki 88921, Japan

[2] Univ Cheikh, Inst Fisheries & Aquaculture, Dakar, Senegal

[3] RIKEN Ctr Sustainable Resource Sci, Drug Discovery Platforms Cooperat Div, Wako, Saitama 3510198, Japan

[4] VIT Univ, Sch Biosci & Technol, Vellore 600014, Tamil Nadu, India

[5] Miyazaki Univ, Interdisciplinary Grad Sch Agr & Engn, Miyazaki 8892192, Japan

[6] Natl Res Inst Aquaculture, Saiki, Oita 8792602, Japan

来源：

TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES | 2014年 / 14卷 / 02期

关键词：

Marsupenaeus japonicus; WSSV; VP28; Innate immune genes; cell-free technique; ENVELOPE PROTEIN VP28; BLACK TIGER SHRIMP; TOLL-LIKE RECEPTORS; SHAPED DNA VIRUS; PENAEUS-MONODON; INNATE IMMUNITY; PROCAMBARUS-CLARKII; ORAL VACCINATION; ANTIMICROBIAL PEPTIDES; LITOPENAEUS-VANNAMEI;

D O I：

10.4194/1303-2712-v14_2_26

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The protective efficacy of oral delivery and intramuscular (i.m.) injection of a recombinant protein, rVP28 derived from white spot syndrome virus (WSSV) and synthesized using wheat germ cell-free technology was investigated in kuruma shrimp (Marsupenaeus japonicus). Shrimps were administered with the unpurified rVP28 and challenged with WSSV. Expression of innate immune-related genes was examined in intestine, heart, and lymphoid organ at 1, 3 and 7 days after oral-administration. Shrimps received rVP28 protein through i.m. injection or oral route and then challenged with WSSV displayed higher survival (90 and 85.7%, respectively) compared to the respective control groups. A significant up-regulation (P<0.01) of innate immune-related genes, such as Rab7, penaeidin, lysozyme and crustin, was noticed in orally treated shrimps. Our results indicate that this recombinant protein could elevate immune responses and protection in kuruma shrimp and therefore would have potential utility against WSSV infection in shrimp aquaculture.

引用

页码：547 / 555

页数：9

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