Electric Field-Driven On-Request Instant in Situ Formation/Removal of Solid Hydrogel within Microchannels for Efficient Electrophoretic Separation

被引：3

作者：

Li, Hongli ^{[1
]}

Li, Fengyun ^{[1
]}

Zhao, Lizhi ^{[1
]}

Zhao, Lei ^{[1
]}

Sun, Ping ^{[1
]}

Wu, Jing ^{[1
]}

Wang, Xiayan ^{[2
]}

Pu, Qiaosheng ^{[1
]}

机构：

[1] Lanzhou Univ, Dept Chem, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Gansu, Peoples R China

[2] Beijing Univ Technol, Beijing Key Lab Green Catalysis & Separat, Dept Chem & Chem Engn, Beijing 100124, Peoples R China

来源：

ACS APPLIED MATERIALS & INTERFACES | 2020年 / 12卷 / 07期

基金：

中国国家自然科学基金;

关键词：

electric field; solid hydrogel; regeneration; electrophoresis; proteins; MICROCHIP ELECTROPHORESIS; CAPILLARY; FLOW; ELECTROCHROMATOGRAPHY; POLYMER; DISPERSION; PROTEINS; CHITOSAN; SERUM; SIZE;

D O I：

10.1021/acsami.9b22878

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Electrophoretic separation in short microchannels is a promising way for rapid analysis of biomolecules, but the pressurized laminar flow may compromise the separation efficiency. In this work, through an electric field, instant formation and removal of a solid chitosan/beta-glycerol phosphate (CS/beta-GP) hydrogel within microchannels of microchips were realized. In a typical cross-type microchip, the CS/beta-GP hydrogel was precisely formed in the separation microchannel within 15 s of the application of a voltage of 2000 V. Highly efficient separation of peptides and proteins was achieved, and theoretical plate numbers of 0.6 to 1.5 x 10(6)/m were attained for proteins in 120 s. The used hydrogel could be swiftly removed also with an electric field, and the whole procedure was achieved on a standard microchip electrophoresis device with no extra accessory or special operation required.

引用

页码：8773 / 8779

页数：7

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