Primary purification of two antifungal proteins from leaves of the fig (Ficus carica L.)

被引：0
|
作者：
Yan, Wei [3 ]
Zhao, Ming [2 ]
Ma, Yan [2 ]
Pan, Ying-hong [1 ]
Yuan, Wen-xia [2 ]
机构：
[1] Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
[2] Yunnan Agr Univ, Coll Pu Erh Tea, Kunming 650201, Peoples R China
[3] Sichuan Normal Univ, Coll Life Sci, Chengdu 610068, Peoples R China
来源：
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2011年 / 10卷 / 03期
关键词：
Fig; antifungal protein; chromatography; natural food preservative; CHITIN-BINDING; IN-VITRO; PEPTIDE; FRUITS; LATEX;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Low-molecular-weight extracts of fig (Ficus carica L.) leaves has antifungal and antibacterial activities against several types of microorganisms. In this work, two high-molecular-weight fractions with antifungal activity, termed figinI and figinII were obtained from leaves of fig using a procedure including ion-exchange chromatography (SP-Sepharose Fast Flow), hydrophobic-interaction chromatography (Phenyl Sepharose 6 Fast Flow and RESOURCE ISO) and ion-exchange chromatography (Mono S). By matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis (MALDI-TOF MS), the molecular mass of figinI was 21531Da and figinII was 31957Da. This is the first report on isolation of antifungal proteins from F. carica L., and it shows their potential for further investigation.
引用
收藏
页码：375 / 379
页数：5
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