A novel method for intratracheal injection of infectious agents into mice

被引：2

作者：

Cheng, Yong ^{[1
,2
,3
]}

Liepert, Lucas D. ^{[1
,4
,5
]}

Waller, Justin ^{[1
,4
,5
]}

Leevy, W. Matthew ^{[1
,4
,5
]}

Schorey, Jeff S. ^{[1
,2
,3
]}

机构：

[1] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA

[2] Univ Notre Dame, Ctr Rare & Neglected Dis, Notre Dame, IN 46556 USA

[3] Univ Notre Dame, Eck Inst Global Hlth, Notre Dame, IN 46556 USA

[4] Univ Notre Dame, Harper Canc Res Ctr, Notre Dame, IN 46556 USA

[5] Univ Notre Dame, Notre Dame Integrated Imaging Facil, Notre Dame, IN 46556 USA

来源：

LABORATORY ANIMALS | 2017年 / 51卷 / 05期

关键词：

intratracheal infection; mice; airborne pathogens; infectious diseases; Mycobacterium tuberculosis; Mycobacterium bovis; 3D printing;

D O I：

10.1177/0023677216684422

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Intratracheal injection is a traditional technique used in small animal studies of highly contagious airborne pathogens such as Mycobacterium tuberculosis. However, current techniques of intratracheal injection generally involve procedures that pose a risk of incident injury and infection for researchers, and may also cause collateral damage to experimental animals during the installation process. Here we describe an intratracheal injection method that was enabled by a three dimensional printing of a custom platform. This updated technique improved the overall ergonomics of intratracheal injection in mice, minimizing the risk of human injury and implementing the 3R (replacement, reduction and refinement) principle in mouse infection studies.

引用

页码：530 / 533

页数：4