Actin expression in Taenia solium cysticerci (cestoda):: tisular distribution and detection of isoforms

被引：19

作者：

Ambrosio, JR ^{[1
]}

Reynoso-Ducoing, O ^{[1
]}

Hernández-Sanchez, H ^{[1
]}

Correa-Piña, D ^{[1
]}

González-Malerva, L ^{[1
]}

Cruz-Rivera, M ^{[1
]}

Flisser, A ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico

来源：

CELL BIOLOGY INTERNATIONAL | 2003年 / 27卷 / 09期

关键词：

actin isoforms; two-dimensional electrophoresis; cysticerci; muscular systems; fluorescence; Taenia solium;

D O I：

10.1016/S1065-6995(03)00142-2

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Identification, localization and partial biochemical characterization of actins expressed in the larval stage of the cestode parasite Taenia solium has been carried out. Frozen tissue sections of cysticerci, the larval stage of this parasite, were reacted with rhodamine-phalloidin, parasite actin was purified by polymerization in the presence of K+, mg(++) and ATP actin was analyzed by SDS-PAGE and two-dimensional gel electrophoresis, and immunoblotting of actin was performed in PVDF membranes and with commercial anti-actin monoclonal antibodies. Parasitic tissues showed different fibrous actin fluorescence patterns, which correlated with the expression of isoactins. Purified globular actin had a similar molecular mass to rabbit commercial actin (similar to44 kDa). Actin was resolved into seven isoforms, indicating a family of actin genes. (C) 2003 Published by Elsevier Ltd.

引用

页码：727 / 733

页数：7

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