The effect of humic acids on the cytotoxicity of silver nanoparticles to a natural aquatic bacterial assemblage

被引:68
作者
Dasari, Thabitha P. [2 ]
Hwang, Huey-Min [1 ,2 ]
机构
[1] Jackson State Univ, Dept Biol, Jackson, MS 39217 USA
[2] Jackson State Univ, Environm Sci PhD Program, Jackson, MS 39217 USA
基金
美国国家科学基金会;
关键词
Silver nanoparticles; Cytotoxicity; Terrestrial humic acid; River humic acid; Reactive oxygen species; 1-AMINOPYRENE ECOTOXICITY; ANTIBACTERIAL ACTIVITY; ESCHERICHIA-COLI; ORGANIC-MATTER; TOXICITY; SUBSTANCES; KINETICS; BEHAVIOR; WATER; ION;
D O I
10.1016/j.scitotenv.2010.08.030
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The effect of a terrestrial humic acid (HA) and a river HA on the cytotoxicity of silver nanoparticles (AgNPs) to natural aquatic bacterial assemblages (0 mu M, 2 5 mu M and 5 mu M) was measured with spread plate counting The effect of HA (20 and 40 ppm) on the cytotoxicity of AgNPs ranging in size between 15 and 25 nm was tested in the presence and in the absence of natural sunlight. The experiment was a full factorial, completely randomized design and the results were analyzed using the General Linear Model in SAS LSMEANS was used to separate the means or combinations of means. Significant main effects of all independent variables, plus interaction effects in all cases except HA/LI and HA/AgNPs/LI were observed The toxicity of AgNPs to natural aquatic bacterial assemblages appears to be concentration dependent for concentrations between 0 mu M and 5 mu M. The data indicate that the light exposure inhibited viability more than the darkness exposure The HA treatment groups in the presence of light showed greater reduced viability count compared to darkness exposure groups The inhibition of bacterial viability counts by AgNPs exposure was less in the light treatment groups containing a terrestrial HA compared to that with a river HA. Difference in the extent of reactive oxygen species formation and adsorption/binding of AgNPs was speculated to account for the observed phenomenon (C) 2010 Elsevier BV All rights reserved
引用
收藏
页码:5817 / 5823
页数:7
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