Genomic instability and increased expression of BUB1B and MAD2L1 genes in ductal breast carcinoma

被引:53
作者
Scintu, Marina
Vitale, Rita
Prencipe, Maria
Gallo, Antometta Pia
Bonghi, Loriana
Valori, Vanna Maria
Maiello, Evaristo
Rinaldi, Monica
Signori, Emanuela
Rabitti, Carla
Carella, Massimo
Dallapiccola, Bruno
Altomare, Vittorio
Fazio, Vito M.
Parrella, Paola
机构
[1] IRCCS Casa Sollievo Sofferenza, Res Dept, Lab Oncol, I-71013 San Giovanni Rotondo, Italy
[2] Univ Bari, Dept Med Biochem Biol & Phys, I-70124 Bari, Italy
[3] IRCCS Casa Sollievo Sofferenza, Dept Oncol, I-71013 San Giovanni Rotondo, Italy
[4] CNR, Inst Neurobiol & Mol Med, Rome, Italy
[5] Mol Med & Biotechnol Lab, I-00155 Rome, Italy
[6] Dept HystoPathol, Rome, Italy
[7] IRCCS Casa Sollievo Sofferenza, Med Genet Lab, I-71013 San Giovanni Rotondo, Italy
[8] Dept Surg, Breast Unit, I-00168 Rome, Italy
关键词
breast cancer; genomic instability; MAD2L1; BUB1B; gene expression;
D O I
10.1016/j.canlet.2007.03.021
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
In a series of invasive ductal breast carcinoma, we investigated the status of chromosomal and intrachromosomal instability by fluorescence in situ hybridisation and determined the level of mRNA expression for two genes involved in the mitotic spindle checkpoint pathway, BUBIB and MAD2L1. All breast cancers demonstrated higher chromosomal instability rates in tumor samples (average: 56.86%, range: 36.24-76.78%) than in controls (average: 11.54%, range: 9.91-14.84%) (P < 0.0001). As well as intrachromosomal instability rates were elevated in tumor (average: 18.45% range: 8.34-35.8%) as compared with controls (average: 4.18% range: 3.47-4.81%) (P < 0.0001). An increase in BUBIB and MAD2L1 transcripts was demonstrated in the majority of the tumor tested. BUBIB mRNA levels but not MAD2L1 levels correlated with intrachromosomal instability (r = 0.722, P = 0.018). (c) 2007 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:298 / 307
页数:10
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