The ham-5, rcm-1 and rco-1 genes regulate hyphal fusion in Neurospora crassa

被引:36
作者
Aldabbous, Mash'el Salman [1 ]
Roca, M. Gabriela [2 ]
Stout, Angela [1 ]
Huang, I-Ching [2 ]
Read, Nick D. [2 ]
Free, Stephen J. [1 ]
机构
[1] SUNY Buffalo, Dept Biol Sci, Buffalo, NY 14260 USA
[2] Univ Edinburgh, Inst Cell Biol, Fungal Cell Biol Grp, Edinburgh EH9 3JH, Midlothian, Scotland
来源
MICROBIOLOGY-SGM | 2010年 / 156卷
基金
英国生物技术与生命科学研究理事会; 美国国家卫生研究院;
关键词
CONIDIAL ANASTOMOSIS TUBES; SEXUAL DEVELOPMENT; MATING-TYPE; PROTEIN; GROWTH; TRANSCRIPTION; TRANSDUCTION; TRICHOGYNES; MORPHOLOGY; FERTILITY;
D O I
10.1099/mic.0.040147-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Mutants of Neurospora crassa unable to participate in vegetative hyphal fusion (anastomosis) were isolated and characterized. From this analysis, three genes, rcm-1, rco-1 and ham-5, were identified and shown to be required for hyphal fusion. The rcm-1 and rco-1 genes are homologues of the Saccharomyces cerevisiae SSN6 and TUP1 genes, which encode a dimeric transcription factor in yeast. We demonstrate that in N. crassa the rcm-1 and rco-1 genes are required for hyphal fusion and normal hyphal morphology, and influence both asexual and sexual development. The ham-5 gene encodes a 1686 amino acid protein with two putative WD40 domains, which might participate in protein-protein interactions. ham-5 deletion mutants had a reduced rate of hyphal extension and altered hyphal morphology, and were unable to produce the conidial anastomosis tubes that are required for hyphal fusion during colony initiation.
引用
收藏
页码:2621 / 2629
页数:9
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