Gene cloning and biochemical characterization of the BMP-2 of Pinctada fucata

被引：41

作者：

Miyashita, Tomoyuki ^{[1
]}

Hanashita, Takuo ^{[1
]}

Toriyama, Michinori ^{[1
]}

Takagi, Ryousuke ^{[1
]}

Akashika, Tamaki ^{[1
]}

Higashikubo, Nobuto ^{[1
]}

机构：

[1] Kinki Univ, Dept Gen Engn, Fac Biol Oriented Sci & Technol, Wakayama 6496493, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2008年 / 72卷 / 01期

关键词：

Pinctada fucata; transforming growth factor type beta (TGF-beta); gene structure; cDNA cloning; biomineralization;

D O I：

10.1271/bbb.70302

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The bone morphogenetic proteins (BMps) constitute a subfamily of the transforming growth factor type beta (TGF-beta) supergene family. BMP-2 plays an important role not only in osteoblast differentiation but also in pattern formation during development. To determine the function of BMP-2 in Pinctada fucata development and hard tissue formation, we isolated a BMP-2 genomic DNA clone and the BMP-2 cDNA. The deduced BMP-2 sequence consisted of 447 amino acids. The BMP-2 gene was composed of three exons. The C-terminal portion (149 amino acids) had 86% and 66% identity to the Crassostrea gigas and the human BMP-2 sequence respectively. The 5' flanking promoter region contained putative glioma transcription factor (Gli) and retinoic acid receptor (RAR) responding elements. The BMP-2 gene was expressed strongly in the inner part of the mantle tissue, corresponding to the nacreous aragonite shell layer. This finding suggests that BMP-2 has a key role in nacreous layer formation.

引用

页码：37 / 47

页数：11

共 51 条

[1] EXPRESSION OF HUMAN BONE MORPHOGENETIC PROTEINS-2 OR PROTEINS-4 IN MURINE MESENCHYMAL PROGENITOR C3H10T1/2 CELLS INDUCES DIFFERENTIATION INTO DISTINCT MESENCHYMAL CELL LINEAGES [J].

AHRENS, M ;

ANKENBAUER, T ;

SCHRODER, D ;

HOLLNAGEL, A ;

MAYER, H ;

GROSS, G .

DNA AND CELL BIOLOGY, 1993, 12 (10) :871-880

[2] Reconstruction of human maxillary defects with nacre powder: histological evidence for bone regeneration [J].

Atlan, G ;

Balmain, N ;

Berland, S ;

Vidal, B ;

Lopez, E .

COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE III-SCIENCES DE LA VIE-LIFE SCIENCES, 1997, 320 (03) :253-258

[3] BMP-4 is proteolytically activated by furin and/or PC6 during vertebrate embryonic development [J].

Cui, YZ ;

Jean, F ;

Thomas, G ;

Christian, JL .

EMBO JOURNAL, 1998, 17 (16) :4735-4743

[4]

DERYNCK R, 1986, J BIOL CHEM, V261, P4377

[5] IDENTIFICATION OF A RETINOIC ACID RESPONSIVE ELEMENT IN THE RETINOIC ACID RECEPTOR-BETA GENE [J].

DETHE, H ;

VIVANCORUIZ, MD ;

TIOLLAIS, P ;

STUNNENBERG, H ;

DEJEAN, A .

NATURE, 1990, 343 (6254) :177-180

[6] Evidence that furin is an authentic transforming growth factor-β1-converting enzyme [J].

Dubois, CM ;

Blanchette, F ;

Laprise, MH ;

Leduc, R ;

Grondin, F ;

Seidah, NG .

AMERICAN JOURNAL OF PATHOLOGY, 2001, 158 (01) :305-316

[7] STRUCTURE AND SEQUENCE OF MOUSE BONE MORPHOGENETIC PROTEIN-2 GENE (BMP-2) - COMPARISON OF THE STRUCTURES AND PROMOTER REGIONS OF BMP-2 AND BMP-4 GENES [J].

FENG, JQ ;

HARRIS, MA ;

GHOSHCHOUDHURY, N ;

FENG, M ;

MUNDY, GR ;

HARRIS, SE .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1994, 1218 (02) :221-224

[8]

FENG JQ, 1995, J BIOL CHEM, V270, P28364, DOI 10.1074/jbc.270.47.28364

[9]

FRUMKIN A, 1993, DEVELOPMENT, V118, P553

[10] RETINOIC ACID RECEPTORS AND CELLULAR RETINOID-BINDING PROTEINS - COMPLEX INTERPLAY IN RETINOID SIGNALING [J].

GIGUERE, V .

ENDOCRINE REVIEWS, 1994, 15 (01) :61-79

← 1 2 3 4 5 6 →