Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen

被引:123
作者
Breininger, E [1 ]
Beorlegui, NB [1 ]
O'Flaherty, CM [1 ]
Beconi, MT [1 ]
机构
[1] Univ Buenos Aires, Area Biochem, Sch Vet Sci, Buenos Aires, DF, Argentina
关键词
cryopreservation; boar spermatozoa; antioxiclants; motility; lipid peroxidation;
D O I
10.1016/j.theriogenology.2004.08.016
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cryopreservation is associated with the production of reactive oxygen species which lead to lipid peroxidation of sperm membranes. The objective was to determine an alpha-tocopherol concentration capable of improving the quality of cryopreserved porcine semen. Boar spermatozoa frozen with 200, 500 or 1000 mu g/mL alpha-tocopherol were thawed and incubated at 37 degrees C for 4 h. Routine parameters of semen quality, susceptibility to lipid peroxidation 2-thiobarbituric acid (TBARS) and oxygen uptake were evaluated. Motility was higher (P < 0.05) in samples treated with different concentrations of alpha-tocopherol up to 2 h of incubation. Viability and acrosome integrity significantly decreased during incubation (no significant differences between treatments). Two hundred micrograms per milliliter alpha-tocopherol protected spermatozoa against lipid peroxidation during incubation, but 1000 mu g/mL failed to protect after 2 h of incubation. There was a negative association between TBARS and motility, suggesting that lipid peroxidation affected sperm motility. Both control and 200 mu g/mL alpha-tocopherol samples preserved the capacity to generate oxidative energy up to 1 h of incubation. The addition of 200 mu g/mL alpha-tocopherol in the semen extender could be useful to preserve boar spermatozoa against the oxidative stress generated by cryopreservation. (c) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:2126 / 2135
页数:10
相关论文
共 36 条
[1]  
AITKEN RJ, 1993, J REPROD FERTIL, V98, P257
[2]   GENERATION OF REACTIVE OXYGEN SPECIES, LIPID-PEROXIDATION, AND HUMAN-SPERM FUNCTION [J].
AITKEN, RJ ;
CLARKSON, JS ;
FISHEL, S .
BIOLOGY OF REPRODUCTION, 1989, 41 (01) :183-197
[3]  
ALVAREZ JG, 1992, J ANDROL, V13, P232
[4]   PHOSPHORYLANT CAPACITY STUDY AND LACTATE MITOCHONDRIAL OXIDATION IN FROZEN BOVINE SPERM [J].
BECONI, MT ;
BEORLEGUI, NB ;
SARMIENTO, NK ;
MORA, NG .
LIFE SCIENCES, 1990, 47 (06) :477-483
[5]  
BECONI MT, 1991, BIOCHEM INT, V23, P545
[6]   EFFECT OF NATURAL ANTIOXIDANTS ON FROZEN BOVINE SEMEN PRESERVATION [J].
BECONI, MT ;
FRANCIA, CR ;
MORA, NG ;
AFFRANCHINO, MA .
THERIOGENOLOGY, 1993, 40 (04) :841-851
[7]  
Beorlegui N, 1997, ANDROLOGIA, V29, P37
[8]   ANTIOXIDANT EFFECT OF VITAMIN-E AND GLUTATHIONE ON LIPID-PEROXIDATION IN BOAR SEMEN PLASMA [J].
BRZEZINSKASLEBODZINSKA, E ;
SLEBODZINSKI, AB ;
PIETRAS, B ;
WIECZOREK, G .
BIOLOGICAL TRACE ELEMENT RESEARCH, 1995, 47 (1-3) :69-74
[9]   HIGH-CONCENTRATIONS OF ANTIOXIDANTS MAY NOT IMPROVE DEFENSE AGAINST OXIDATIVE STRESS [J].
CAO, GH ;
CUTLER, RG .
ARCHIVES OF GERONTOLOGY AND GERIATRICS, 1993, 17 (03) :189-201
[10]   Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage [J].
Cerolini, S ;
Maldjian, A ;
Surai, P ;
Noble, R .
ANIMAL REPRODUCTION SCIENCE, 2000, 58 (1-2) :99-111