SYBR Green I-based real-time PCR targeting the rpoX gene for sensitive and rapid detection of Vibrio alginolyticus

被引：15

作者：

Zhao Jing-jing ^{[1
,2
]}

Chen Chang ^{[1
]}

Luo Peng ^{[1
]}

Ren Chun-hue ^{[1
]}

Jiang Xiao ^{[1
,2
]}

Zhao Zhe ^{[1
]}

Hu Chao-qun ^{[1
]}

机构：

[1] CAS, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China

[2] CAS, Grad Univ, Beijing 100039, Peoples R China

来源：

MOLECULAR AND CELLULAR PROBES | 2011年 / 25卷 / 2-3期

基金：

美国国家科学基金会;

关键词：

Real-time PCR; rpoX gene; Vibrio alginolyticus; SEQUENCE-ANALYSIS; SHRIMP; PARAHAEMOLYTICUS; IDENTIFICATION; CHOLERAE; STRAINS; GYRB; TOXR;

D O I：

10.1016/j.mcp.2011.02.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

rpoX, a Vibrio alginolyticus specific stress regulating gene, was used to detect this fish pathogen by SYBR Green I-based real-time PCR. The specificity of the detection was confirmed in different samples. The minimum level of detection was 10(3) cells from pure culture and 10(2) cells from seawater. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：137 / 141

页数：5

共 19 条

[1] Investigation of several virulence properties among Vibrio alginolyticus strains isolated from diseased cultured fish in Tunisia [J].

Ben Kahla-Nakbi, Amel ;

Chaieb, Kamel ;

Bakhrouf, Amina .

DISEASES OF AQUATIC ORGANISMS, 2009, 86 (01) :21-28

[2] A novel multiplex PCR method for detecting virulent strains of Vibrio alginolyticus [J].

Cai, Shuang-Hu ;

Lu, Yi-Shan ;

Wu, Zao-He ;

Jian, Ji-Chang ;

Huang, Yuang-Cong .

AQUACULTURE RESEARCH, 2009, 41 (01) :27-34

[3] Phenotypic and genetic differences between opaque and translucent colonies of Vibrio alginolyticus [J].

Chen Chang ;

Xie Jin ;

Hu Chaoqun .

BIOFOULING, 2009, 25 (06) :525-531

[4] A collagenase-targeted multiplex PCR assay for identification of Vibrio alginolyticus, Vibrio cholerae, and Vibrio parahaemolyticus [J].

Di Pinto, A ;

Ciccarese, G ;

Tantillo, G ;

Catalano, D ;

Forte, VT .

JOURNAL OF FOOD PROTECTION, 2005, 68 (01) :150-153

[5] Genetic heterogeneity among Vibrio alginolyticus isolated from shrimp farms by PCR fingerprinting [J].

George, MR ;

John, KR ;

Iyappan, T ;

Jeyaseelan, MJP .

LETTERS IN APPLIED MICROBIOLOGY, 2005, 40 (05) :369-372

[6] Quantification of Vibrio penaeicida, the etiological agent of Syndrome 93 in New Caledonian shrimp, by real-time PCR using SYBR Green I chemistry [J].

Goarant, Cyrille ;

Merien, Fabrice .

JOURNAL OF MICROBIOLOGICAL METHODS, 2006, 67 (01) :27-35

[7] Isolation of Vibrio alginolyticus and Vibrio splendidus from aquacultured carpet shell clam (Ruditapes decussatus) larvae associated with mass mortalities [J].

Gómez-León, J ;

Villamil, L ;

Lemos, ML ;

Novoa, B ;

Figueras, A .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (01) :98-104

[8] Characterization of a Vibrio alginolyticus strain, isolated from Alaskan oysters, carrying a hemolysin gene similar to the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus [J].

Gonzalez-Escalona, Narjol ;

Blackstone, George M. ;

DePaola, Angelo .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (12) :7925-7929

[9]

[胡学峰 Hu Xuefeng], 2005, [中国海洋大学学报. 自然科学版, Journal of Ocean University of China], V35, P232

[10] Application of the rpoS Gene for Species-Specific Detection of Vibrio vulnificus by Real-Time PCR [J].

Kim, Dong-Gyun ;

Ahn, Sun-Hee ;

Kim, Lyoung-Hwa ;

Park, Kee-Jai ;

Hong, Yong-Ki ;

Kong, In-Soo .

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 18 (11) :1841-1847

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