Methods of simultaneous preparation of various active substances from Stichopus chloronotus and functional evaluation of active substances

Stichopus chloronotus, a marine sea cucumber rich in n-3 PUFAs and phenolic compounds, is a deep black-green sea cucumber with yellow/ red papillae tips, which has important economic and ecological value. In this project, a process was established to simultaneous extract and prepare four different active substances (including sea cucumber polysaccharide, polypeptide, saponin and lipid) from Stichopus chloronotus. The yields and purity were also improved. Except for polysaccharides, the purity of the other three active substances was above 90%. The extraction rates were all above 60%. In addition, the functional activity of sea cucumber peptides was evaluated. The results showed that sea cucumber peptides could reduce triglyceride content in mice serum, increase glutathione content and relieve alcohol-induced liver damage. The technical research laid a technical foundation for the high value utilization of Stichopus chloronotus.