Preliminary x-ray crystallographic analysis of centrin from ciliate Euplotes octocarinatus

被引：1
作者：
He, XJ
Li, L
Hou, H
Zhao, YQ
Liang, AH
Rao, ZH [1 ]
机构：
[1] Tsinghua Univ, Struct Biol Lab, Beijing 100084, Peoples R China
[2] Chinese Acad Sci, China & Natl Lab Macromol, Inst Biophys, Beijing 100101, Peoples R China
[3] Shanxi Univ, Inst Biotechnol, Key Lab Chem Biol & Mol Engn, Minist Educ, Yaiyuan 030006, Peoples R China
来源：
PROTEIN AND PEPTIDE LETTERS | 2005年 / 12卷 / 06期
关键词：
centrin; crystallization; preliminary X-ray studies;
D O I：
10.2174/0929866054395842
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Centrins are four-EF-hand Ca2+-binding proteins, which belong to the CaM super family. The centrin from ciliate Euplotes Octocarinatus has been expressed in Escherichia coli, purified and crystallized using the hanging-drop method. Rod-like crystals were grown and diffracted to 2.0 angstrom. The crystals belong to space group P2(1)2(1)2(1) and the unit-cell parameters are a=34.442 angstrom, b=48.954 angstrom, c=72.583 angstrom.
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收藏
页码：609 / 611
页数：3
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