Enhanced activity of the Gmar MT1 promoter from the mycorrhizal fungus Gigaspora margarita at limited carbon supply

被引:3
作者
Bergero, Roberta
Lanfranco, Luisa
Ghignone, Stefano
Bonfante, Paola
机构
[1] Scottish Agr Coll, Crop & Soil Res Grp, Edinburgh EH9 3JG, Midlothian, Scotland
[2] Univ Turin, Dipartimento Biol Vegetale, Turin, Italy
[3] CNR, Sez Torino, Ist Protez Piante, I-10125 Turin, Italy
关键词
plant symbiosis; carbon metabolism; life cycle; mycorrhiza; metallothionein; UAS;
D O I
10.1016/j.fgb.2007.01.010
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Metal lothioneins are low molecular weight polypeptides, present in most eukaryotic phyla, with role in metal homeostasis and detoxification. We previously reported the identification and the characterization of a metallothionein gene (GmarMT1) from the arbuscular mycorrhizal fungus Gigaspora margarita. Here, we have used real-time quantitative RT-PCR to show that GinarMT1 expression was turned off during the symbiotic fungal growth in the hexose-rich mycorrhizal apoplast, whereas transcripts were abundant during all other fungal growth stages, when products of lipid breakdown and the glyoxylate cycle feed carbohydrate-consuming pathways. In support of a nutritional regulation of GmarMT1 expression, we show that transcriptional activity of GmarMT1 promoter in yeast was strongly induced by glucose starvation (up to 20 times the basal level). We speculate that GmarMT1-encoded protein, with its proved metal binding ability, could regulate the homeostasis of zinc, a fundamental cofactor involved in C metabolism regulation and glucose repression. (C) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:877 / 885
页数:9
相关论文
共 57 条
[31]  
Lammers PJ, 2001, PLANT PHYSIOL, V127, P1287, DOI 10.1104/pp.010375
[32]   The mycorrhizal fungus Gigaspora margarita possesses a CuZn superoxide dismutase that is up-regulated during symbiosis with legume hosts [J].
Lanfranco, L ;
Novero, M ;
Bonfante, P .
PLANT PHYSIOLOGY, 2005, 137 (04) :1319-1330
[33]   Differential expression of a metallothionein gene during the presymbiotic versus the symbiotic phase of an arbuscular mycorrhizal fungus [J].
Lanfranco, L ;
Bolchi, A ;
Ros, EC ;
Ottonello, S ;
Bonfante, P .
PLANT PHYSIOLOGY, 2002, 130 (01) :58-67
[34]   GintAMT1 encodes a functional high-affinity ammonium transporter that is expressed in the extraradical mycelium of Glomus intraradices [J].
López-Pedrosa, A ;
González-Guerrero, M ;
Valderas, A ;
Azcón-Aguilar, C ;
Ferrol, N .
FUNGAL GENETICS AND BIOLOGY, 2006, 43 (02) :102-110
[35]  
Maniatis T.A., 1982, MOL CLONING
[36]   The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress-response element (STRE) [J].
MartinezPastor, MT ;
Marchler, G ;
Schuller, C ;
MarchlerBauer, A ;
Ruis, H ;
Estruch, F .
EMBO JOURNAL, 1996, 15 (09) :2227-2235
[37]  
MUNGER K, 1987, J BIOL CHEM, V262, P7363
[38]   Dual requirement of the LjS']jSym4 gene for mycorrhizal development in epidermal and cortical cells of Lotus japonicus roots [J].
Novero, M ;
Faccio, A ;
Genre, A ;
Stougaard, J ;
Webb, KJ ;
Mulder, L ;
Parniske, M ;
Bonfante, P .
NEW PHYTOLOGIST, 2002, 154 (03) :741-749
[39]  
PAGANI A, 2006, MOL MICROBIOL
[40]   The elusive function of metallothioneins [J].
Palmiter, RD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (15) :8428-8430