Chromosome segregation control by Escherichia coli ObgE GTPase

被引:40
作者
Foti, James J.
Persky, Nicole S.
Ferullo, Daniel J.
Lovett, Susan T.
机构
[1] Brandeis Univ, Dept Biol, Waltham, MA 02454 USA
[2] Brandeis Univ, Rosenstiel Basic Med Sci Res Ctr, Waltham, MA 02454 USA
关键词
DIVISION SITE SELECTION; BINDING PROTEIN OBG; ACTIN-LIKE PROTEINS; BACILLUS-SUBTILIS; CELL-DIVISION; STRINGENT RESPONSE; DNA-REPLICATION; MREB; FTSZ; SEQA;
D O I
10.1111/j.1365-2958.2007.05811.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Escherichia coli cells depleted of the conserved GTPase, ObgE, show early chromosome-partitioning defects and accumulate replicated chromosomes in which the terminus regions are colocalized. Cells lacking ObgE continue to initiate replication, with a normal ratio of the origin to terminus. Localization of the SeqA DNA binding protein, normally seen as punctate foci, however, was disturbed. Depletion of ObgE also results in cell filamentation, with polyploid DNA content. Depletion of ObgE did not cause lethality, and cells recovered fully after expression of ObgE was restored. We propose a model in which ObgE is required to license chromosome segregation and subsequent cell cycle events.
引用
收藏
页码:569 / 581
页数:13
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