Aspidin BB, a phloroglucinol derivative, exerts its antibacterial activity against Staphylococcus aureus by inducing the generation of reactive oxygen species

被引:33
作者
Li, Na [1 ]
Gao, Chang [2 ]
Peng, Xiao [1 ]
Wang, Wei [1 ]
Luo, Meng [1 ]
Fu, Yu-jie [1 ]
Zu, Yuan-gang [1 ]
机构
[1] Northeast Forestry Univ, Minist Educ, Engn Res Ctr Forest Biopreparat, Harbin 150040, Peoples R China
[2] Peking Univ, Hlth Sci Center, Coll Basic Med Sci, Beijing 100083, Peoples R China
关键词
Dryopteris fragrans; Aspidin BB; Reactive oxygen species; Membrane; DNA; Protein; OXIDATIVE STRESS; ANTIMICROBIAL ACTIVITY; ESCHERICHIA-COLI; FREE-RADICALS; NANOPARTICLES; INACTIVATION; ANTIOXIDANTS; DRYOFRAGIN; MORPHOLOGY; ARECOLINE;
D O I
10.1016/j.resmic.2014.03.002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Aspidin BB, a phloroglucinol derivative extracted from Dryopteris fragrans (L.) Schott, has been previously reported to exert high biological activities. In the present study, antibacterial activities and mechanisms of aspidin BB against Staphylococcus aureus were investigated. Aspidin BB exhibited strong antibacterial activity against standard and three clinical S. aureus, with minimal inhibition concentration (MIC) values ranging from 15.63 mu g/mL to 62.5 mu g/mL. After treatment with aspidin BB for 72 h using the MTT assay, the IC50 value was 48.14 mu M (22.11 mu g/mL). The time kill assay indicated that aspidin BB could kill S. aureus completely at 2 MIC (MBC) within 4 h. Aspidin BB was capable to induce an increase in NADPH oxidase activity from 4.03 U/mg to 7.48 U/mg when the concentrations were increased from 1/2 MIC to 4 MIC. Simultaneously, aspidin BB attenuated antioxidant defense by decreasing superoxide dismutase (SOD) activity and glutathione (GSH) levels. The level of reactive oxygen species (ROS) was apparently elevated when measuring OD575. This phenomenon was blocked by pretreatment of S. aureus with the antioxidant compound catalase (200 U/mL) and the survival rate of S. aureus increased from 3.95% to 73.04%. These results showed that ROS was indeed an important mediator in the antibacterial action of aspidin BB. In addition, aspidin-BB-induced peroxidation of membranes, DNA damage and protein degradation of S. aureus were all verified in a dose-dependent manner. In conclusion, aspidin BB induced generation of ROS by activating NADPH oxidase activity and inhibiting SOD activity and GSH levels, damaging the membrane, DNA and proteins and finally led to cell death. (C) 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:263 / 272
页数:10
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