High genetic diversity of porcine enterovirus G in Schleswig-Holstein, Germany

被引:37
作者
Bunke, Jennifer [1 ,2 ]
Receveur, Kerstin [1 ,2 ]
Oeser, Ann Christin [1 ,2 ]
Fickenscher, Helmut [1 ,2 ]
Zell, Roland [3 ,4 ]
Krumbholz, Andi [1 ,2 ]
机构
[1] Christian Albrechts Univ Kiel, Inst Infekt Med, Brunswiker Str 4, D-24105 Kiel, Germany
[2] Univ Klinikum Schleswig Holstein, Brunswiker Str 4, D-24105 Kiel, Germany
[3] Friedrich Schiller Univ Jena, Inst Med Mikrobiol, Sekt Expt Virol, Hans Knoll Str 2, D-07743 Jena, Germany
[4] Univ Klinikum Jena, Hans Knoll Str 2, D-07743 Jena, Germany
关键词
MOLECULAR CHARACTERIZATION; WILD BOARS; TESCHOVIRUSES; DISEASE; THURINGIA; FEATURES; STRAIN; PIGS;
D O I
10.1007/s00705-017-3612-x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Between 2012 and 2015, 495 pooled snout swabs from fattening pigs raised in Schleswig-Holstein, Germany, were screened for the presence of enterovirus G (EV-G) RNA. Nucleic acids were tested in diverse reverse transcription polymerase chain reaction assays applying published oligonucleotide primers specific for the viral protein (VP) 1 and 2/4 encoding regions as well as for 3D polymerase. Phylogenetic analyses of VP1 revealed the presence of 12 EV-G types, three of which had highly divergent sequences suggesting putative new types. Co-circulation of EV-G types was observed in several pigsties. Thus, genetic diversity of EV-G was demonstrated in this small geographic area.
引用
收藏
页码:489 / 493
页数:5
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