Biocompatibility and Antibacterial Action of Salvadora persica Extract as Intracanal Medication (In Vitro and Ex Vivo Experiment)

被引:7
作者
Abdeltawab, Samah Samir [1 ]
Abu Haimed, Tariq S. [2 ]
Bahammam, Hammam Ahmed [3 ]
Arab, Wafaa Talat [4 ]
Abou Neel, Ensanya A. [5 ,6 ,7 ]
Bahammam, Laila Ahmed [8 ]
机构
[1] King Abdulaziz Univ, Endodont Dept, Jeddah 21589, Saudi Arabia
[2] King Abdulaziz Univ, Operat Dent, Jeddah 21589, Saudi Arabia
[3] King Abdulaziz Univ, Dept Pediat Dent, Jeddah 21589, Saudi Arabia
[4] King Abdulaziz Univ, King Fahd Med Res Ctr, Jeddah 21589, Saudi Arabia
[5] Univ Sharjah, Coll Dent Med, Prevent & Restorat Dent Dept, Sharjah 27272, U Arab Emirates
[6] Tanta Univ, Fac Dent, Biomat Dept, Tanta 31527, Egypt
[7] Royal Free Hosp, UCL Eastman Dent Inst, Biomat & Tissue Engn, Rowland Hill St, London NW32QG, England
[8] King Abdulaziz Univ, Fac Dent, Dept Endodont, Jeddah 21589, Saudi Arabia
关键词
S; persica; miswak; Ca(OH)(2); E; faecalis; intracanal medication; arak roots; ENTEROCOCCUS-FAECALIS; CALCIUM HYDROXIDE; ENDODONTIC INFECTIONS; EFFICACY; MEDICAMENTS; ROTARY; CHLORHEXIDINE; DISINFECTION; PROPOLIS; BACTERIA;
D O I
10.3390/ma15041373
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
This study aimed to test the biocompatibility and antibacterial properties of Salvadora persica (S. persica) extract, a natural product, as an intracanal medication in comparison with calcium hydroxide (Metapaste, META BIOMED, Cheongju, Korea). The pH values of both materials were tested. The biocompatibility of S. persica extract and Metapaste was determined using light microscopy and MTT assays. The antibacterial action was tested using the zone of bacterial inhibition on four common bacterial species. In addition, intracanal medication was administered using 68 extracted single-rooted teeth contaminated with Enterococcus faecalis (E. faecalis), and the percentage reduction in colony count (% RCC) at 1, 3, and 7 days was measured. The extension of activity for both materials was assessed using histological sections and scanning electron microscopy. S. persica was found to be acidic in nature. Both materials showed significantly lower cell viability than the positive control cells on days 1 and 3 but not on day 7. S. persica showed better antibacterial effects against E. faecalis and S. mutans. S. persica extract showed 97.6%, 98.9%, and 99.3% RCC values at 1, 3, and 7 days, respectively, which are comparable to those of Metapaste. S. persica herbal extract is a promising material that can be utilized as an intracanal medication, but its use requires further research.
引用
收藏
页数:19
相关论文
共 69 条
[1]  
Abdallah E., 2015, J PHYTOPHARMACOLOGY, V4, P243, DOI [10.31254/phyto.2015.4501, DOI 10.31254/PHYTO.2015.4501]
[2]   Antibiofilm efficacy of silver nanoparticles as a vehicle for calcium hydroxide medicament against Enterococcus faecalis [J].
Afkhami, Farzaneh ;
Pourhashemi, Seyyed Jalal ;
Sadegha, Mona ;
Salehi, Yasaman ;
Fard, Mohammad Javad Kharrazi .
JOURNAL OF DENTISTRY, 2015, 43 (12) :1573-1579
[3]  
Aguiar Alana Souza, 2015, J Contemp Dent Pract, V16, P624
[4]   A review on phytochemical and pharmacological investigations of miswak (Salvadora persica Linn) [J].
Akhtar, Jamal ;
Siddique, Khalid M. ;
Bi, Salma ;
Mujeeb, Mohd .
JOURNAL OF PHARMACY AND BIOALLIED SCIENCES, 2011, 3 (01) :113-117
[5]   Antibacterial Activity of Salvadora persica L. (Miswak) Extracts against Multidrug Resistant Bacterial Clinical Isolates [J].
Al-Ayed, Mohamed Saeed Zayed ;
Asaad, Ahmed Morad ;
Qureshi, Mohamed Ansar ;
Attia, Hany Goda ;
AlMarrani, Abduljabbar Hadi .
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2016, 2016
[6]  
Al-Bayati FA, 2008, TURK J BIOL, V32, P57
[7]  
Al-Nimer MSM, 2017, J INTERCULT ETHNOPHA, V6, P280, DOI 10.5455/jice.20170630092831
[8]   An in vitro and in vivo antibacterial effect of different plant extracts on Enterococcus faecalis as intracanal medicament [J].
Al-Sabawi, Nawal A. ;
Al-Naimi, Ali M. ;
Yahya, Eman M. .
JOURNAL OF INTERNATIONAL ORAL HEALTH, 2020, 12 (04) :362-369
[9]  
Al-sabawi NawalA.K., 2007, University of Sharjah Journal of Pure Applied Sciences, V4, P69
[10]   THE ANTIMICROBIAL EFFECT OF VARIOUS ENDODONTIC SEALERS [J].
ALKHATIB, ZZ ;
BAUM, RH ;
MORSE, DR ;
YESILSOY, C ;
BHAMBHANI, S ;
FURST, ML .
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS, 1990, 70 (06) :784-790