Inhibition of translational initiation by Let-7 microRNA in human cells

被引：1047
|
作者：
Pillai, RS
Bhattacharyya, SN
Artus, CG
Zoller, T
Cougot, N
Basyuk, E
Bertrand, E
Filipowicz, W [1 ]
机构：
[1] Friedrich Miescher Inst Biomed Res, CH-4002 Basel, Switzerland
[2] Inst Genet Mol Montpellier, F-34000 Montpellier, France
来源：
SCIENCE | 2005年 / 309卷 / 5740期
关键词：
D O I：
10.1126/science.1115079
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
MicroRNAs (miRNAs) are similar to 21-nucteotide-long RNA molecules regulating gene expression in multicellular eukaryotes. In metazoa, miRNAs act by imperfectly base-pairing with the 3' untranslated region of target messenger RNAs (mRNAs) and repressing protein accumulation by an unknown mechanism. We demonstrate that endogenous let-7 microribonucleoproteins (miRNPs) or the tethering of Argonaute (Ago) proteins to reporter mRNAs in human cells inhibit translation initiation. M(7)G-cap-independent translation is not subject to repression, suggesting that miRNPs interfere with recognition of the cap. Repressed mRNAs, Ago proteins, and miRNAs were all found to accumulate in processing bodies. We propose that localization of mRNAs to these structures is a consequence of translational repression.
引用
收藏
页码：1573 / 1576
页数：4
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