Coordinated expression of clustered cancer/testis genes encoded in a large inverted repeat DNA structure

被引：13

作者：

Bredenbeck, Anne ^{[1
,2
]}

Hollstein, Verena M. ^{[1
]}

Trefzer, Uwe ^{[1
]}

Sterry, Wolfram ^{[1
]}

Walden, Peter ^{[1
]}

Losch, Florian O. ^{[1
]}

机构：

[1] Charite Univ Med Berlin, Dept Dermatol, Clin Res Grp Tumor Immunol, Skin Canc Ctr Charite, D-10098 Berlin, Germany

[2] Free Univ Berlin, Dept Biol Chem & Pharm, D-14195 Berlin, Germany

来源：

GENE | 2008年 / 415卷 / 1-2期

关键词：

cancer/testis antigens; MAGE; CSAG; double-cruciform DNA structure; chromatin structure;

D O I：

10.1016/j.gene.2008.02.019

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Cancer/testis antigens (CTA) are expressed in cancers and testis or placenta only and, therefore are considered promising targets for cancer immunotherapy and diagnosis. One family of CTA is the MAGEA family which comprises 13 members and was shown to be expressed synchronously with members from the CSAG (TRAG-3) family of CTA. The MAGEA genes are arranged in 4 subdusters located on the X chromosome. Subcluster III exposes a remarkable gene organization with an inverted repeat (IR) DNA structure of a triplicated couplet of a MAGEA gene and a CSAG gene. Analyzing the mRNA expression pattern of all genes of the MAGEA and CSAG family of cancer/testis genes, we show that the MAGEA and CSAG genes encoded in the large IR are expressed coordinately and independent from the MAGEAs encoded outside the IR. These results reinforce our hypothesis that the large MAGEA/CSAG-IR DNA structure has an impact on the regulation of gene expression. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：68 / 73

页数：6

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