THE EFFECTS OF HYALURONIC ACID, CALCIUM HYDROXIDE, AND DENTIN ADHESIVE ON RAT ODONTOBLASTS AND FIBROBLASTS

被引：16
作者：
Bogovic, Ana
Nizetic, Jana
Galic, Nada [1 ]
Zeljezic, Davor [2 ]
Micek, Vedran [2 ]
Mladinic, Mann [2 ]
机构：
[1] Univ Zagreb, Sch Dent Med, Dept Endodont & Restorat Stomatol, Zagreb 10000, Croatia
[2] Univ Zagreb, Inst Med Res & Occupat Hlth, Zagreb 10000, Croatia
来源：
ARHIV ZA HIGIJENU RADA I TOKSIKOLOGIJU-ARCHIVES OF INDUSTRIAL HYGIENE AND TOXICOLOGY | 2011年 / 62卷 / 02期
关键词：
direct pulp capping; fibroblast proliferation; odontoblastic proliferation; HUMAN PULP CELLS; IN-VITRO; BONDING AGENTS; CYTOTOXICITY; SYSTEM; APOPTOSIS; HEMA; DENTINOGENESIS; COMPONENTS; INDUCTION;
D O I：
10.2478/10004-1254-62-2011-2076
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
The aim of this study was to investigate the effects and efficiency of pulp capping preparations based on hyaluronic acid, calcium hydroxide, and dentin adhesive on the pulp tissue of Sprague-Dawley rats. The rats were killed and extracted teeth sectioned transversely through the pulp. The slices were placed in a RPMI 1640 cell culture medium supplemented with 10% foetal calf serum. During 14 days of cultivation cultures were treated with preparations that contained hyaluronic acid (Gengigel Prof (R)), and calcium hydroxide (ApexCar (R)), or with dentin adhesive (Excite (R)). Cellularity and viability of fibroblasts and odontoblasts was analysed using a haemocytometer. Hyaluronic acid proved most efficient and the least toxic for direct pulp capping. Even though calcium hydroxide and dentin adhesive demonstrated a higher degree of cytotoxicity, their effects were still acceptable in terms of biocompatibility.
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页码：155 / 161
页数：7
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