3D Culture of Chondrocytes in Gelatin Hydrogels with Different Stiffness

被引：182

作者：

Li, Xiaomeng ^{[1
,2
]}

Chen, Shangwu ^{[1
,2
]}

Li, Jingchao ^{[1
,2
]}

Wang, Xinlong ^{[1
,2
]}

Zhang, Jing ^{[1
,2
]}

Kawazoe, Naoki ^{[1
]}

Chen, Guoping ^{[1
,2
]}

机构：

[1] Natl Inst Mat Sci, Int Ctr Mat Nanoarchitecton, 1-1 Namiki, Tsukuba, Ibaraki 3050044, Japan

[2] Univ Tsukuba, Grad Sch Pure & Appl Sci, Dept Mat Sci & Engn, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058577, Japan

来源：

POLYMERS | 2016年 / 8卷 / 08期

关键词：

gelatin; hydrogel; stiffness; chondrogenic phenotype; tissue engineering; STEM-CELL DIFFERENTIATION; MATRIX ELASTICITY; PORE-SIZE; CYTOSKELETAL; BIOMATERIALS; DEGRADATION; MAINTENANCE; POLYMERS; SCAFFOLD; ALGINATE;

D O I：

10.3390/polym8080269

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Gelatin hydrogels can mimic the microenvironments of natural tissues and encapsulate cells homogeneously, which makes them attractive for cartilage tissue engineering. Both the mechanical and biochemical properties of hydrogels can affect the phenotype of chondrocytes. However, the influence of each property on chondrocyte phenotype is unclear due to the difficulty in separating the roles of these properties. In this study, we aimed to study the influence of hydrogel stiffness on chondrocyte phenotype while excluding the role of biochemical factors, such as adhesion site density in the hydrogels. By altering the degree of methacryloyl functionalization, gelatin hydrogels with different stiffnesses of 3.8, 17.1, and 29.9 kPa Young's modulus were prepared from the same concentration of gelatin methacryloyl (GelMA) macromers. Bovine articular chondrocytes were encapsulated in the hydrogels and cultured for 14 days. The influence of hydrogel stiffness on the cell behaviors including cell viability, cell morphology, and maintenance of chondrogenic phenotype was evaluated. GelMA hydrogels with high stiffness (29.9 kPa) showed the best results on maintaining chondrogenic phenotype. These results will be useful for the design and preparation of scaffolds for cartilage tissue engineering.

引用

页数：15

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