Smartphone Fluorescence Spectroscopy

被引：1

作者：

Yu, Hojeong ^{[1
]}

Tan, Yafang ^{[1
]}

Cunningham, Brian T. ^{[1
,2
]}

机构：

[1] Univ Illinois, Dept Elect & Comp Engn, Micro & Nanotechnol Lab, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Bioengn, Micro & Nanotechnol Lab, Urbana, IL 61801 USA

来源：

FRONTIERS IN BIOLOGICAL DETECTION: FROM NANOSENSORS TO SYSTEMS VII | 2015年 / 9310卷

关键词：

spectroscopy; fluorescence; smartphone detection; Forster resonance energy transfer; FRET; QUANTUM DOTS; DNA; BIOMOLECULES; ASSAY;

D O I：

10.1117/12.2083999

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

We demonstrate the first use of smartphone spectrophotometry for readout of fluorescence-based biological assays. We evaluated the smartphone fluorimeter in the context of a fluorescent molecular beacon (MB) assay for detection of a specific nucleic acid sequences in a liquid test sample. The capability of distinguishing a one-point mismatch is also demonstrated by detecting single-base mutation in target nucleic acids. Our approach offers a route towards portable biomolecular assays for viral/bacterial pathogens, disease biomarkers, and toxins.

引用

页数：4

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