Non-covalent modification of the active site of cytochrome P450 for inverting the stereoselectivity of monooxygenation

被引：19

作者：

Fujishiro, Takashi ^{[1
]}

Shoji, Osami ^{[1
]}

Watanabe, Yoshihito ^{[1
,2
]}

机构：

[1] Nagoya Univ, Grad Sch Sci, Dept Chem, Chikusa Ku, Nagoya, Aichi 4648602, Japan

[2] Nagoya Univ, Res Ctr Mat Sci, Chikusa Ku, Nagoya, Aichi 4648602, Japan

来源：

TETRAHEDRON LETTERS | 2011年 / 52卷 / 03期

关键词：

Cytochrome P450; Stereoselectivity; Sulfoxidation; Protein modification; Hydrogen peroxide; Biocatalyst; BACILLUS-SUBTILIS; SUBSTRATE RECOGNITION; ENANTIOSELECTIVITY; LIPASE; INVERSION; MUTATION; ENZYMES;

D O I：

10.1016/j.tetlet.2010.11.048

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The enantioselectivity in the sulfoxidation of thioanisole catalyzed by cytochrome P450(BS beta) with a decoy molecule, a dummy molecule of the natural substrate, can be inverted by changing the structure of the decoy molecule. The methodology demonstrated herein shows the potential for controlling the stereoselectivity of biocatalysts without any mutagenesis. (C) 2010 Elsevier Ltd. All rights reserved.

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收藏

页码：395 / 397

页数：3

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