Genetic and Environmental Controls on Nitrous Oxide Accumulation in Lakes

被引：56

作者：

Saarenheimo, Jatta ^{[1
]}

Rissanen, Antti J. ^{[1
]}

Arvola, Lauri ^{[2
]}

Nykanen, Hannu ^{[1
]}

Lehmann, Moritz F. ^{[3
]}

Tiirola, Marja ^{[1
]}

机构：

[1] Univ Jyvaskyla, Dept Biol & Environm Sci, Jyvaskyla 40014, Finland

[2] Univ Helsinki, Lammi Biol Stn, Lammi 16900, Finland

[3] Univ Basel, Dept Environm Sci, CH-4058 Basel, Switzerland

来源：

PLOS ONE | 2015年 / 10卷 / 03期

基金：

芬兰科学院;

关键词：

DENITRIFYING BACTERIA; MICROBIAL COMMUNITY; NATURAL WATERS; RIBOSOMAL-RNA; END-PRODUCT; NOSZ GENES; DENITRIFICATION; NITRATE; SOILS; N2O;

D O I：

10.1371/journal.pone.0121201

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We studied potential links between environmental factors, nitrous oxide (N2O) accumulation, and genetic indicators of nitrite and N2O reducing bacteria in 12 boreal lakes. Denitrifying bacteria were investigated by quantifying genes encoding nitrite and N2O reductases (nirS/nirK and nosZ, respectively, including the two phylogenetically distinct clades nosZ(I) and nosZ(II)) in lake sediments. Summertime N2O accumulation and hypolimnetic nitrate concentrations were positively correlated both at the inter-lake scale and within a depth transect of an individual lake (Lake Vanajavesi). The variability in the individual nirS, nirK, nosZ(I), and nosZ(II) gene abundances was high (up to tenfold) among the lakes, which allowed us to study the expected links between the ecosystem's nir-vs-nos gene inventories and N2O accumulation. Inter-lake variation in N2O accumulation was indeed connected to the relative abundance of nitrite versus N2O reductase genes, i.e. the (nirS+nirK)/nosZ(I) gene ratio. In addition, the ratios of (nirS+ nirK)/nosZ(I) at the inter-lake scale and (nirS+ nirK)/nosZ(I+II) within Lake Vanajavesi correlated positively with nitrate availability. The results suggest that ambient nitrate concentration can be an important modulator of the N2O accumulation in lake ecosystems, either directly by increasing the overall rate of denitrification or indirectly by controlling the balance of nitrite versus N2O reductase carrying organisms.

引用

页数：14

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