Extraction of testosterone and epitestosterone in human urine using aqueous two-phase systems of ionic liquid and salt

被引:24
|
作者
He, CY [1 ]
Li, SH [1 ]
Liu, HW [1 ]
Li, K [1 ]
Liu, F [1 ]
机构
[1] Peking Univ, Minist Educ, Coll Chem, Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China
基金
中国国家自然科学基金;
关键词
extraction; ionic liquid; aqueous two-phase systems; testosterone; epitestosterone; human urine;
D O I
10.1016/j.chroma.2005.05.065
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Based on aqueous two-phase systems (ATPS) consisting of 1-butyl-3-methylimidazolium chloride, a hydrophilic ionic liquid (IL), and K2HPO4, a new and simple extraction technique, coupled with a reversed-phase high performance liquid chromatography (RP-HPLC), was developed for the simultaneous concentration and analysis of testosterone (T) and epitestosterone (ET) in human urine. Under the optimal conditions, the extraction efficiencies for both analytes were 80-90% in a one-step extraction. The method required only 3.0 mL of urine and a single hydrolysis/deproteinization/extraction step followed by direct injection of the IL-rich upper phase into HPLC system for analysis. The method has been satisfactorily applied to the analysis of T and ET in human urine with detection limits of I ng/mL and linear ranges of 10-500 ng/mL for both compounds. Compared with conventional liquid-liquid extraction or solid phase extraction, this new method is much "greener" due to no use of volatile organic solvent and low consumption of IL. The proposed extraction technique opens up new possibilities in the separation of other drugs. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:143 / 149
页数:7
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