Molecular characterization of hospital-acquired methicillin-resistant Staphylococcus aureus strains in pediatric outbreaks using variable tandem repeat analysis with spa and ClfB typing

被引：12

作者：

Ugolotti, Elisabetta ^{[1
]}

Bandettini, Roberto ^{[1
]}

Marchese, Anna ^{[2
]}

Gualco, Laura ^{[2
]}

Vanni, Irene ^{[1
]}

Borzi, Luana ^{[1
]}

Di Marco, Eddi ^{[1
]}

Castagnola, Elio ^{[3
]}

Melioli, Giovanni ^{[1
]}

Biassoni, Roberto ^{[1
]}

机构：

[1] Ist Giannina Gaslini, Dept Expt Med, I-16147 Genoa, Italy

[2] Univ Genoa, Di SCMIT, Microbiol Sect, I-16132 Genoa, Italy

[3] Ist Giannina Gaslini, Dept Hematol & Oncol, Infect Dis Unit, I-16147 Genoa, Italy

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2011年 / 69卷 / 02期

关键词：

MRSA; Sequence typing; Repeat assay analysis; clfB; S. aureus protein A; PROTEIN-A GENE; FIELD GEL-ELECTROPHORESIS; CLONES;

D O I：

10.1016/j.diagmicrobio.2010.08.015

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

To analyze 67 clinical methicillin-resistant Staphylococcus aureus (MRSA) isolated from pediatric hospital infections, we used multilocus variable-number tandem-repeat DNA sequence-based techniques, targeting the protein A polymorphic X region and the clumping factor B complete R domain. We define a "clfB similarity score" and then compare the double loci analysis of closely related MRSA isolates with pulsed-field gel electrophoresis (PFGE). We found an endemic clone (MLST-ST8, spa-t008, SCCmecIV, ClfB lineage 1) able to originate 3 possible outbreaks and a second clone (MLST-ST152, spa-t355, SCCmecV, CHB lineage 4) responsible for limited cases of MRSA infections, indicating that the combination of spa and clfB-lineage typing is useful to trace MRSA pediatric outbreaks. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：213 / 217

页数：5

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