Advanced genome-editing technologies enable rapid and large-scale generation of genetic variants for strain engineering and synthetic biology

被引：11

作者：

Zeng, Yi ^{[1
]}

Hong, Yuxiang ^{[1
]}

Azi, Fidelis ^{[1
]}

Liu, Yugeng ^{[1
]}

Chen, Yousheng ^{[1
]}

Guo, Chuchu ^{[1
]}

Lin, Dewei ^{[1
]}

Wu, Zizhao ^{[1
]}

Chen, Wenhao ^{[1
]}

Xu, Peng ^{[1
,2
,3
]}

机构：

[1] Guangdong Technion Israel Inst Technol GTIIT, Dept Chem Engn, Shantou 515063, Guangdong, Peoples R China

[2] Guangdong Technion Israel Inst Technol, Guangdong Prov Key Lab Mat & Technol Energy Conve, Shantou 515063, Guangdong, Peoples R China

[3] Technion Israel Inst Technol, Wolfson Dept Chem Engn, IL-32000 Haifa, Israel

来源：

CURRENT OPINION IN MICROBIOLOGY | 2022年 / 69卷

关键词：

SITE-SPECIFIC RECOMBINATION; NUCLEIC-ACID DETECTION; HOMOLOGOUS RECOMBINATION; PRECISE MANIPULATION; CRE RECOMBINASE; DNA; CHROMOSOMES; CRISPR-CAS12A; REPLACEMENT; MUTAGENESIS;

D O I：

10.1016/j.mib.2022.102175

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Targeted genome editing not only improves our understanding of fundamental rules in life sciences but also affords us versatile toolkits to improve industrially relevant phenotypes in various host cells. In this review, we summarize the recent endeavor to develop efficient genome-editing tools, and emphasize the utility of these tools to generate massive scale of genetic variants. We categorize these tools into traditional recombination-based tools, and more advanced CRISPR as well as RNA-based genome-editing tools. This diverse panel of sophisticated tools has been applied to accelerate strain engineering, upgrade biomanufacturing, and customize biosensing. In parallel with high-throughput phenotyping and AI-based optimization algorithms, we envision that genome-editing technologies will become a driving force to automate and streamline biological engineering, and empower us to address critical challenges in health, environment, energy, and sustainability.

引用

页数：12

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