New insights into the anti-erosive property of a sugarcane-derived cystatin: different vehicle of application and potential mechanism of action

被引:6
作者
Gironda, Carlos Condarco [1 ]
Pela, Vinicius Taioqui [2 ]
Henrique-Silva, Flavio [2 ]
Delbem, Alberto Carlos Botazzo [3 ]
Pessan, Juliano Pelim [3 ]
Buzalaf, Marilia Afonso Rabelo [1 ]
机构
[1] Univ Sao Paulo, Fac Odontol Bauru, Dept Ciencias Biol, Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP, Brazil
[2] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Carlos, SP, Brazil
[3] Univ Estadual Paulista, Fac Odontol Aracatuba, Dept Odontol Prevent & Restauradora, Sao Paulo, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
Acquired pellicle; Tooth erosion; Cystatin; SURFACE FREE-ENERGY; ACQUIRED PELLICLE; IN-VITRO; SALIVA; WETTABILITY; FLUORIDE;
D O I
10.1590/1678-7757-2021-0698
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
A new sugarcane-derived cystatin (CaneCPI-5) showed anti-erosive properties when included in solutions and strong binding force to enamel, but the performance of this protein when added to gel formulations and its effect on surface free energy (SFE) requires further studies. Objective: 1) to evaluate the protective effect of gels containing different concentrations of CaneCPI-5 against initial enamel erosion (Experiment 1); and 2) to analyze the SFE (gamma(S)) after treating the enamel surface with CaneCPI-5 solution (Experiment 2). Methodology: In Experiment 1, 75 bovine enamel specimens were divided into five groups according to the gel treatments: placebo (negative control); 0.27%mucin+0.5%casein (positive control); 0.1 mg/ mL CaneCPI-5; 1.0 mg/mL CaneCPI-5; or 2.0 mg/mL CaneCPI-5. Specimens were treated with the gels for 1 min, the AP was formed (human saliva) for 2 h and the specimens were incubated in 0.65% citric acid (pH=3.4) for 1 min. The percentage of surface hardness change (%SHC) was estimated. In Experiment 2, measurements were performed by an automatic goniometer using three probing liquids: diiodomethane, water and ethylene glycol. Specimens (n=10/group) remained untreated (control) or were treated with solution containing 0.1 mg/ mL CaneCPI-5, air-dried for 45 min, and 0.5 mu L of each liquid was dispensed on the surface to measure contact angles. Results: Gels containing 0.1 and 1.0 mg/ mL CaneCPI-5 significantly reduced %SHC compared to the other treatments (p<0.05). Treated enamel showed significantly lower g S than control, without changes in the apolar component (gamma(LW)(S)), but the polar component (gamma(AB)(S)=Lewis acidbase) became more negative (p<0.01). Moreover, CaneCPI-5 treatment showed higher gamma(S) - (electron-donor) values compared to control (p<0.01). Conclusions: Gels containing 0.1 mg/mL or 1.0 mg/mL CaneCPI-5 protected enamel against initial dental erosion. CaneCPI-5 increased the number of electron donor sites on the enamel surface, which may affect AP formation and could be a potential mechanism of action to protect from erosion.
引用
收藏
页数:7
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