Two-Photon Absorbing Dyes with Minimal Autofluorescence in Tissue Imaging: Application to in Vivo Imaging of Amyloid-β Plaques with a Negligible Background Signal

被引：180

作者：

Kim, Dokyoung ^{[1
]}

Moon, Hyunsoo ^{[1
]}

Baik, Sung Hoon ^{[2
]}

Singha, Subhankar ^{[1
]}

Jun, Yong Woong ^{[1
]}

Wang, Taejun ^{[3
]}

Kim, Ki Hean ^{[3
]}

Park, Byung Sun ^{[4
]}

Jung, Junyang ^{[4
]}

Mook-Jung, Inhee ^{[2
]}

Ahn, Kyo Han ^{[1
]}

机构：

[1] Pohang Univ Sci & Technol POSTECH, Ctr Electrobehav Adv Mol Syst, Dept Chem, Pohang 790784, Gyungbuk, South Korea

[2] Seoul Natl Univ, Coll Med, Dept Biochem & Biomed Sci, Seoul 110799, South Korea

[3] Pohang Univ Sci & Technol POSTECH, Div Integrat Biosci & Biotechnol, Pohang 790784, Gyungbuk, South Korea

[4] Kyung Hee Univ, Sch Med, Inst Biomed Sci, Dept Anat & Neurobiol, Seoul 130701, South Korea

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2015年 / 137卷 / 21期

基金：

新加坡国家研究基金会;

关键词：

FLUORESCENT-PROBES; MULTIPHOTON MICROSCOPY; ACIDIC VESICLES; TRANSGENIC MICE; LIPID RAFTS; LIVE CELLS; CONGO RED; IONS; STATES; SPECTROSCOPY;

D O I：

10.1021/jacs.5b03548

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Fluorescence imaging of tissues offer an essential means for studying biological systems. Autofluorescence becomes a serious issue in tissue imaging under excitation at UV-vis wavelengths where biological molecules compete with the fluorophore. To address this critical issue, a novel class of fluorophores that can :be excited at, similar to 900 nm under two-photon excitation conditions and emits in the red wavelength region (>= 600 nm) has been disclosed. The new pi-extended dipolar dye system, shows several advantageous features including minimal antofluorescence in tissue imaging and pronounced solvent-sensitive emission behavior, compared with a widely used two-photon absorbing dye, acedan. As an important application of the new dye system, one of the dyes was developed into a fluorescent probe for amyloicl-beta plaques, a key biomarker of Alzheimer's disease. The probe enabled in Vivo imaging of amyloid-beta plaques in a disease-model mouse, with negligible background signal. The new dye system has great potential for the development of other types of two-photon fluorescent probes and tags for imaging of tissues with minimal autofluorescence.

引用

页码：6781 / 6789

页数：9

共 73 条

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