Influence of growth regulators on distribution of trichomes and the production of volatiles in micropropagated plants of Plectranthus ornatus

被引:13
|
作者
Passinho-Soares, Helna C. [1 ,2 ]
David, Juceni P. [1 ]
de Santana, Jose R. F. [2 ]
David, Jorge M. [3 ]
Rodrigues, Frederico de M. [4 ]
Mesquita, Paulo R. R. [3 ]
de Oliveira, Fabio S. [5 ]
Bellintani, Moema C. [6 ]
机构
[1] Univ Fed Bahia, Lab Pesquisa Prod Nat, Fac Farm, Salvador, BA, Brazil
[2] Univ Estadual Feira de Santana, Programa Posgrad Biotecnol, Feira De Santana, BA, Brazil
[3] Univ Fed Bahia, Inst Quim, Salvador, BA, Brazil
[4] Empresa Baiana Desenvolvimento Agr SA, Salvador, BA, Brazil
[5] Univ Fed Reconcavo Baiano, Ctr Ciencias Saude, Santo Antonio De Jesus, BA, Brazil
[6] Univ Fed Bahia, Inst Biol, Salvador, BA, Brazil
来源
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY | 2017年 / 27卷 / 06期
关键词
In vitro cultivation; Trichomes; Plant growth regulators; VOC; Monoterpenes; GLANDULAR TRICHOMES; ESSENTIAL OIL; CHEMICAL-COMPOSITION; L; LEAVES; GRANDIDENTATUS; CONSTITUENTS; DITERPENOIDS; LABDANE; ANATOMY;
D O I
10.1016/j.bjp.2017.10.001
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
The profile of volatile organic compounds, the glandular and non-glandular trichomes of Plectranthus ornatus, obtained by in vitro cultivation, was evaluated in plants grown in Murashide and Skoog medium supplemented with benylaminopurine at 4.5, 9.0, and 18.0 mu M + naphthaleneacetic acid at 5.37 mu M, kinetin at 4.7, 9.3 and 18.5 mu M + naphthaleneacetic acid (5.37 mu M) or Murashide and Skoog 0 medium (as a control). Scanning Electron Microscopy was performed on samples of the third leaf node of the 90 days old plants obtained from treatment with 4.5 or 9.0 mu M benylaminopurine, and 4.7 or 9.3 mu M kinetin.Headspace Solid Phase Micro-Extraction of the 30, 60 and 90 days old in vitro plants permitted to determinate by GC/MS the composition comprised of 62 compounds. The data were analyzed using Principal Component Analysis and Hierarchical Clustering Analysis and, the major constituents of these oils after treatment and aging were monoterpenes and sesquiterpenes. Morphoanatomical analysis of trichomes, by Scanning Electron Microscopy, enabled the identification of non-glandular trichomes and four types of glandular trichomes, which comprised capitate and peltate glandular trichomes that were distributed on both sides of the leaf. We observed that the regulators influenced qualitative and quantitative profiles of the volatile organic compounds and the number and distribution of hairs on the leaf surface. (c) 2017 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.
引用
收藏
页码:679 / 690
页数:12
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