NAD biosynthesis: Identification of the tryptophan to quinolinate pathway in bacteria

被引：170
作者：
Kurnasov, O
Goral, V
Colabroy, K
Gerdes, S
Anantha, S
Osterman, A
Begley, TP
机构：
[1] Cornell Univ, Dept Chem & Chem Biol, Ithaca, NY 14853 USA
[2] Integrated Genom, Chicago, IL 60612 USA
来源：
CHEMISTRY & BIOLOGY | 2003年 / 10卷 / 12期
关键词：
D O I：
10.1016/j.chembiol.2003.11.011
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Previous studies have demonstrated two different bio-synthetic pathways to quinolinate, the universal de novo precursor to the pyridine ring of NAD. In prokaryotes, quinolinate is formed from aspartate and dihydroxyacetone phosphate; in eukaryotes, it is formed from tryptophan. It has been generally believed that the tryptophan to quinolinic acid biosynthetic pathway is unique to eukaryotes; however, this paper describes the use of comparative genome analysis to identify likely candidates for all five genes involved in the tryptophan to quinolinic acid pathway in several bacteria. Representative examples of each of these genes were overexpressed, and the predicted functions are confirmed in each case using unambiguous biochemical assays.
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页码：1195 / 1204
页数：10
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