Physico-chemical characterization of bovine serum albumin-cationic gemini surfactant interaction

Being perspective candidates for bioapplications, gemini surfactant - protein interaction research is continuing extensively. In the work described herein, we have, therefore, investigated the binding of a recently synthesized biodegradable cationic diester-functionalized C-14-E2O2-C-14 gemini surfactant with ethylene oxide group in the spacer with the transport protein bovine serum albumin (BSA). The system properties were determined through a combination of surface tension measurements, multispectroscopic and computational analyses. The surface and interfacial properties of the concerned gemini got perturbed on interaction with BSA. The reduction in BSA fluorescence intensity was accredited to fluorescence quenching by the surfactant and relevant parameters (K-SV and K-b; 14.70, 10.63, 7.27 x 10(4) M-1 and 25.63, 14.28, 7.20 x 10(4) M-1) have been computed at three different temperatures (288, 298 and 308 K), which demonstrated that C-14-E2O2-C-14 binds strongly with BSA protein through static quenching (UV-vis absorption analysis results were the same) with the binding stoichiometry 1:1. The obtained negative values of thermodynamic parameters (Delta G(b)(0), Delta H-b(0) and Delta S-b(0)) specified spontaneous process where hydrogen bonding and van der Waals forces are the major forces in the interaction mechanism. The effect of beta-cyclodextrin (beta-CD) has also been explored on the interaction and the values of K-SV and K-b have been evaluated. Moreover, some other spectral results (CD, 3-D, synchronous, RRS, pyrene, FT-IR) confirmed that the interaction of the surfactant with BSA persuaded alterations in conformation of BSA. The molecular docking results as well as site probe measurements corroborated the ligand binding sites and interaction of the gemini surfactant with amino acid residues. The binding analysis of C-14-E2O2-C-14 gemini surfactant-BSA interaction is potentially valuable for elucidating chemico-biological interaction that can be used in the pharmaceuticals, biochemistry and pharmacology arenas. (C) 2022 Elsevier B.V. All rights reserved.