Hsa_Circ_0007843 Acts as a mIR-518c-5p Sponge to Regulate the Migration and Invasion of Colon Cancer SW480 Cells
被引:34
作者:
He, Jin Hua
论文数: 0引用数: 0
h-index: 0
机构:
Cent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R ChinaCent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R China
He, Jin Hua
[1
]
Han, Ze Ping
论文数: 0引用数: 0
h-index: 0
机构:
Cent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R ChinaCent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R China
Han, Ze Ping
[1
]
Luo, Jin Gen
论文数: 0引用数: 0
h-index: 0
机构:
Cent Hosp Panyu Dist, Digesting Ctr, Guangzhou, Peoples R ChinaCent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R China
Luo, Jin Gen
[2
]
Jiang, Jian Wei
论文数: 0引用数: 0
h-index: 0
机构:
Jinan Univ, Med Coll, Dept Biochem, Guangzhou, Peoples R ChinaCent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R China
Jiang, Jian Wei
[3
]
Zhou, Jia Bin
论文数: 0引用数: 0
h-index: 0
机构:
Cent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R ChinaCent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R China
Zhou, Jia Bin
[1
]
Chen, Wei Ming
论文数: 0引用数: 0
h-index: 0
机构:
Cent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R ChinaCent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R China
Chen, Wei Ming
[1
]
Lv, Yu Bing
论文数: 0引用数: 0
h-index: 0
机构:
Cent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R ChinaCent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R China
Lv, Yu Bing
[1
]
He, Meng Ling
论文数: 0引用数: 0
h-index: 0
机构:
Cent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R ChinaCent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R China
He, Meng Ling
[1
]
Zheng, Lei
论文数: 0引用数: 0
h-index: 0
机构:
Southern Med Univ, Nanfang Hosp, Dept Lab Med, Guangzhou, Peoples R ChinaCent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R China
Zheng, Lei
[4
]
Li, Yu Guang
论文数: 0引用数: 0
h-index: 0
机构:
Cent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R ChinaCent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R China
Li, Yu Guang
[1
]
Zuo, Ji Dong
论文数: 0引用数: 0
h-index: 0
机构:
Sun Yat Sen Univ, Affiliated Hosp 1, Dept Gastrointestinal Surg, Guangzhou, Peoples R ChinaCent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R China
Zuo, Ji Dong
[5
]
机构:
[1] Cent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R China
[2] Cent Hosp Panyu Dist, Digesting Ctr, Guangzhou, Peoples R China
[3] Jinan Univ, Med Coll, Dept Biochem, Guangzhou, Peoples R China
[4] Southern Med Univ, Nanfang Hosp, Dept Lab Med, Guangzhou, Peoples R China
[5] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Gastrointestinal Surg, Guangzhou, Peoples R China
Circular RNA (circRNA), a type of RNA that is widely expressed in mammalian cells, is considered to be essential in tumorigenesis. CircRNA can regulate target gene expression by interacting with the corresponding microRNA (miRNA). Our preliminary results showed that the expression levels of 1,817 circRNAs were significantly different in colon cancer tissue compared with paracancerous tissue, of which 1,236 were upregulated and 581 were downregulated. By using RT-PCR, we confirmed that the expression of hsa_circ_0007843, hsa_circ_0010575, hsa_circ_0007331, and hsa_circ_0001615 was significantly higher in colon cancer tissue than in normal colonic tissue; however, the expression levels of hsa_circ_0014879 and hsa_circRNA_401801 were not significantly different between normal and neoplastic colonic tissue. Among the circRNAs that were confirmed to be upregulated in colon cancer tissue, hsa_circ_0007843 was also found to be highly expressed in colon cancer SW480 cells. Overexpression of hsa_circ_0007843 promoted the invasion and migration of SW480 cells, whereas its downregulation suppressed their invasion and migration. Overexpression of hsa_circ_0007843 promoted tumor growth, whereas its downregulation inhibited tumor growth. We found that hsa_circ_0007843 interacted with miR-518c-5p and suppressed its expression, and miR-518c-5p interacted with matrix metallopeptidase 2 (MMP2) and promoted its expression and translation. Taken together, this study demonstrated that hsa_circ_0007843 acted as an miRNA sponge to regulate MMP2 expression by removing the inhibitory effect of miR-518c-5p on MMP2 gene translation, which further affected the invasive capability of SW480 cells.
机构:
Biodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, SpainBiodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, Spain
Arnaiz, Esther
;
Sole, Carla
论文数: 0引用数: 0
h-index: 0
机构:
Biodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, SpainBiodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, Spain
Sole, Carla
;
Manterola, Lorea
论文数: 0引用数: 0
h-index: 0
机构:
Biodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, SpainBiodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, Spain
Manterola, Lorea
;
Iparraguirre, Leire
论文数: 0引用数: 0
h-index: 0
机构:
Biodonostia Res Inst, Multiple Sclerosis Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, SpainBiodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, Spain
Iparraguirre, Leire
;
Otaegui, David
论文数: 0引用数: 0
h-index: 0
机构:
Biodonostia Res Inst, Multiple Sclerosis Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, SpainBiodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, Spain
Otaegui, David
;
Lawrie, Charles H.
论文数: 0引用数: 0
h-index: 0
机构:
Biodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, Spain
Univ Oxford, John Radcliffe Hosp, Radcliffe Dept Med, Oxford OX3 9DU, England
Ikerbasque, Basque Fdn Sci, Maria Diaz Haroko Kalea 3, Bilbao 48013, SpainBiodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, Spain
机构:
Mackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Mackay Med Nursing & Management Coll, Nursing Div, Taipei, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Hsu, Hsi-Hsien
;
Kuo, Wei-Wen
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Dept Biol Sci & Technol, Taichung, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Kuo, Wei-Wen
;
Day, Cecilia Hsuan
论文数: 0引用数: 0
h-index: 0
机构:
Mei Ho Univ, Dept Nursing, Pingguang Rd, Pingtung, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Day, Cecilia Hsuan
;
论文数: 引用数:
h-index:
机构:
Shibu, Marthandam Asokan
;
Li, Shin-Yi
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Grad Inst Basic Med Sci, Taichung, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Li, Shin-Yi
;
Chang, Sheng-Huang
论文数: 0引用数: 0
h-index: 0
机构:
Tsao Tun Psychiat Ctr, Dept Hlth, Execut Yuan, Nantou, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Chang, Sheng-Huang
;
Shih, Hui-Nung
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Grad Inst Basic Med Sci, Taichung, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Shih, Hui-Nung
;
Chen, Ray-Jade
论文数: 0引用数: 0
h-index: 0
机构:
Taipei Med Univ, Sch Med, Dept Surg, Coll Med, Taipei, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Chen, Ray-Jade
;
Viswanadha, Vijaya Padma
论文数: 0引用数: 0
h-index: 0
机构:
Bharathiar Univ, Dept Biotechnol, Coimbatore 641046, Tamil Nadu, IndiaMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Viswanadha, Vijaya Padma
;
Kuo, Yueh-Hsiung
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Dept Chinese Pharmaceut Sci & Chinese Med Resourc, Taichung, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Kuo, Yueh-Hsiung
;
Huang, Chih-Yang
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Grad Inst Basic Med Sci, Taichung, Taiwan
China Med Univ, Grad Inst Chinese Med Sci, Taichung, Taiwan
Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
机构:
Biodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, SpainBiodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, Spain
Arnaiz, Esther
;
Sole, Carla
论文数: 0引用数: 0
h-index: 0
机构:
Biodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, SpainBiodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, Spain
Sole, Carla
;
Manterola, Lorea
论文数: 0引用数: 0
h-index: 0
机构:
Biodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, SpainBiodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, Spain
Manterola, Lorea
;
Iparraguirre, Leire
论文数: 0引用数: 0
h-index: 0
机构:
Biodonostia Res Inst, Multiple Sclerosis Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, SpainBiodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, Spain
Iparraguirre, Leire
;
Otaegui, David
论文数: 0引用数: 0
h-index: 0
机构:
Biodonostia Res Inst, Multiple Sclerosis Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, SpainBiodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, Spain
Otaegui, David
;
Lawrie, Charles H.
论文数: 0引用数: 0
h-index: 0
机构:
Biodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, Spain
Univ Oxford, John Radcliffe Hosp, Radcliffe Dept Med, Oxford OX3 9DU, England
Ikerbasque, Basque Fdn Sci, Maria Diaz Haroko Kalea 3, Bilbao 48013, SpainBiodonostia Res Inst, Mol Oncol Grp, Paseo Doctor Begiristain S-N, San Sebastian 20014, Spain
机构:
Mackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Mackay Med Nursing & Management Coll, Nursing Div, Taipei, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Hsu, Hsi-Hsien
;
Kuo, Wei-Wen
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Dept Biol Sci & Technol, Taichung, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Kuo, Wei-Wen
;
Day, Cecilia Hsuan
论文数: 0引用数: 0
h-index: 0
机构:
Mei Ho Univ, Dept Nursing, Pingguang Rd, Pingtung, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Day, Cecilia Hsuan
;
论文数: 引用数:
h-index:
机构:
Shibu, Marthandam Asokan
;
Li, Shin-Yi
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Grad Inst Basic Med Sci, Taichung, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Li, Shin-Yi
;
Chang, Sheng-Huang
论文数: 0引用数: 0
h-index: 0
机构:
Tsao Tun Psychiat Ctr, Dept Hlth, Execut Yuan, Nantou, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Chang, Sheng-Huang
;
Shih, Hui-Nung
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Grad Inst Basic Med Sci, Taichung, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Shih, Hui-Nung
;
Chen, Ray-Jade
论文数: 0引用数: 0
h-index: 0
机构:
Taipei Med Univ, Sch Med, Dept Surg, Coll Med, Taipei, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Chen, Ray-Jade
;
Viswanadha, Vijaya Padma
论文数: 0引用数: 0
h-index: 0
机构:
Bharathiar Univ, Dept Biotechnol, Coimbatore 641046, Tamil Nadu, IndiaMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Viswanadha, Vijaya Padma
;
Kuo, Yueh-Hsiung
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Dept Chinese Pharmaceut Sci & Chinese Med Resourc, Taichung, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
Kuo, Yueh-Hsiung
;
Huang, Chih-Yang
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Grad Inst Basic Med Sci, Taichung, Taiwan
China Med Univ, Grad Inst Chinese Med Sci, Taichung, Taiwan
Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung, TaiwanMackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan