Hsa_Circ_0007843 Acts as a mIR-518c-5p Sponge to Regulate the Migration and Invasion of Colon Cancer SW480 Cells

被引:34
作者
He, Jin Hua [1 ]
Han, Ze Ping [1 ]
Luo, Jin Gen [2 ]
Jiang, Jian Wei [3 ]
Zhou, Jia Bin [1 ]
Chen, Wei Ming [1 ]
Lv, Yu Bing [1 ]
He, Meng Ling [1 ]
Zheng, Lei [4 ]
Li, Yu Guang [1 ]
Zuo, Ji Dong [5 ]
机构
[1] Cent Hosp Panyu Dist, Dept Lab Med, Guangzhou, Peoples R China
[2] Cent Hosp Panyu Dist, Digesting Ctr, Guangzhou, Peoples R China
[3] Jinan Univ, Med Coll, Dept Biochem, Guangzhou, Peoples R China
[4] Southern Med Univ, Nanfang Hosp, Dept Lab Med, Guangzhou, Peoples R China
[5] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Gastrointestinal Surg, Guangzhou, Peoples R China
关键词
hsa_circ_0007843; matrix metallopeptidase 2; miR-518c-5p; colon cancer; invasion; migration; CIRCULAR RNA; COLORECTAL-CANCER; BIOMARKER; EXPRESSION;
D O I
10.3389/fgene.2020.00009
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Circular RNA (circRNA), a type of RNA that is widely expressed in mammalian cells, is considered to be essential in tumorigenesis. CircRNA can regulate target gene expression by interacting with the corresponding microRNA (miRNA). Our preliminary results showed that the expression levels of 1,817 circRNAs were significantly different in colon cancer tissue compared with paracancerous tissue, of which 1,236 were upregulated and 581 were downregulated. By using RT-PCR, we confirmed that the expression of hsa_circ_0007843, hsa_circ_0010575, hsa_circ_0007331, and hsa_circ_0001615 was significantly higher in colon cancer tissue than in normal colonic tissue; however, the expression levels of hsa_circ_0014879 and hsa_circRNA_401801 were not significantly different between normal and neoplastic colonic tissue. Among the circRNAs that were confirmed to be upregulated in colon cancer tissue, hsa_circ_0007843 was also found to be highly expressed in colon cancer SW480 cells. Overexpression of hsa_circ_0007843 promoted the invasion and migration of SW480 cells, whereas its downregulation suppressed their invasion and migration. Overexpression of hsa_circ_0007843 promoted tumor growth, whereas its downregulation inhibited tumor growth. We found that hsa_circ_0007843 interacted with miR-518c-5p and suppressed its expression, and miR-518c-5p interacted with matrix metallopeptidase 2 (MMP2) and promoted its expression and translation. Taken together, this study demonstrated that hsa_circ_0007843 acted as an miRNA sponge to regulate MMP2 expression by removing the inhibitory effect of miR-518c-5p on MMP2 gene translation, which further affected the invasive capability of SW480 cells.
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页数:12
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