Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays

被引:6
作者
Cassaniti, Irene [1 ,2 ]
Ferrari, Alessandro [1 ]
Comolli, Giuditta [1 ,3 ]
Sarasini, Antonella [1 ]
Gregorini, Marilena [4 ,5 ]
Rampino, Teresa [4 ,5 ]
Lilleri, Daniele [1 ]
Baldanti, Fausto [1 ,2 ]
机构
[1] IRCCS Policlin San Matteo, Microbiol & Virol Dept, Mol Virol Unit, I-27100 Pavia, Italy
[2] Univ Pavia, Dept Clin Surg Diagnost & Pediat Sci, I-27100 Pavia, Italy
[3] Fdn IRCCS Policlin San Matteo, Lab Biochem Biotechnol & Adv Diagnost, I-27100 Pavia, Italy
[4] Fdn IRCCS Policlin San Matteo, Unit Nephrol Dialysis & Transplantat, I-27100 Pavia, Italy
[5] Univ Pavia, Dept Internal Med & Therapeut, I-27100 Pavia, Italy
关键词
varicella-zoster virus; T effector and T central memory response; transplant; KIDNEY-TRANSPLANTATION; VIRUS; IMMUNITY; VACCINATION; DYSFUNCTION; LYMPHOCYTES; INFECTION;
D O I
10.3390/vaccines9080875
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Solid organ transplant recipients, due to the administration of post-transplant immunosuppressive therapies, are at greater risk of viral reactivation episodes, mainly from herpes viruses, including varicella-zoster virus (VZV). The aim of this pilot study was to develop functional immunological assays (VZV-ELISpot) for the quantification and characterization of the VZV-specific effector-memory and central-memory responses in healthy subjects and transplanted patients. Glycoprotein gE and immediate-early 63 (IE-63) were used as antigens for in vitro stimulation. VZV-seropositive healthy subjects showed higher responses in respect to seronegative subjects. Even if differences were observed between VZV-seropositive healthy subjects and transplanted subjects at pre-transplant, the VZV-specific T-cell response was reduced at 60 days after transplant, mainly for the high level of immunosuppression. Phenotypical characterization revealed that response against VZV was mainly mediated by CD4 T cells. The results obtained in this study might be useful for the definition of personalized follow-up of the transplanted patients, providing useful information on the status of the patient potentially at risk of viral reactivation or other opportunistic infections.
引用
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页数:10
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