5,6-Dehydrokawain from Alpinia zerumbet promotes osteoblastic MC3T3-E1 cell differentiation

被引：23

作者：

Kumagai, Momochika ^{[1
,2
]}

Mishima, Takashi ^{[1
]}

Watanabe, Akio ^{[1
]}

Harada, Teppei ^{[1
]}

Yoshida, Izumi ^{[1
]}

Fujita, Kazuhiro ^{[1
]}

Watai, Masatoshi ^{[1
]}

Tawata, Shinkichi ^{[3
]}

Nishikawa, Keisuke ^{[2
]}

Morimoto, Yoshiki ^{[2
]}

机构：

[1] Japan Food Res Labs, Osaka, Japan

[2] Osaka City Univ, Grad Sch Sci, Dept Chem, Osaka, Japan

[3] Univ Ryukyus, Dept Biosci & Biotechnol, Fac Agr, Nishihara, Okinawa, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2016年 / 80卷 / 07期

关键词：

Alpinia zerumbet; 5; 6-dehydrokawain; MC3T3-E1; osteoblast; osteoporosis; BONE-FORMATION; ALKALINE-PHOSPHATASE; UP-REGULATION; EXPRESSION; EXTRACT; KINASE; P38; MINERALIZATION; PROLIFERATION; OSTEOPOROSIS;

D O I：

10.1080/09168451.2016.1153959

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bone homeostasis is maintained by balancing bone formation and bone resorption, but an imbalance between them is associated with various bone-related diseases such as osteoporosis and rheumatoid arthritis. We found that 5,6-dehydrokawain (DK) and dihydro-5,6-dehydrokawain (DDK), which were isolated as promising compounds from Alpinia zerumbet rhizomes, promote differentiation of osteoblastic MC3T3-E1 cells. DK and DDK increased the alkaline phosphatase activity and matrix mineralization of MC3T3-E1 cells. DK exerts larger effects than DDK. The gene expression of runt-related transcription factor 2 and osterix, which are essential transcription factors in the early period of osteoblast differentiation, was significantly increased by DK treatment. The mRNA level of distal-less homeobox 5 was also enhanced by DK treatment, and DK activated the p38 mitogen-activated protein kinase pathway. Therefore, DK may have clinical potential for preventing osteoporosis, and could be considered as a potential anabolic therapeutic agent.

引用

页码：1425 / 1432

页数：8

共 39 条

[1] Growth Arrest by the Antitumor Steroidal Lactone Withaferin A in Human Breast Cancer Cells Is Associated with Down-regulation and Covalent Binding at Cysteine 303 of β-Tubulin [J].

Antony, Marie L. ;

Lee, Joomin ;

Hahm, Eun-Ryeong ;

Kim, Su-Hyeong ;

Marcus, Adam I. ;

Kumari, Vandana ;

Ji, Xinhua ;

Yang, Zhen ;

Vowell, Courtney L. ;

Wipf, Peter ;

Uechi, Guy T. ;

Yates, Nathan A. ;

Romero, Guillermo ;

Sarkar, Saumendra N. ;

Singh, Shivendra V. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289 (03) :1852-1865

[2] An NMR Spectroscopic Method to Identify and Classify Thiol-Trapping Agents: Revival of Michael Acceptors for Drug Discovery? [J].

Avonto, Cristina ;

Taglialatela-Scafati, Orazio ;

Pollastro, Federica ;

Minassi, Alberto ;

Di Marzo, Vincenzo ;

De Petrocellis, Luciano ;

Appendino, Giovanni .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 50 (02) :467-471

[3] Osteoclast differentiation and activation [J].

Boyle, WJ ;

Simonet, WS ;

Lacey, DL .

NATURE, 2003, 423 (6937) :337-342

[4] Effect of Alpinia zerumbet components on antioxidant and skin diseases-related enzymes [J].

Chompoo, Jamnian ;

Upadhyay, Atul ;

Fukuta, Masakazu ;

Tawata, Shinkichi .

BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2012, 12

[5] Advanced glycation end products inhibitors from Alpinia zerumbet rhizomes [J].

Chompoo, Jamnian ;

Upadhyay, Atul ;

Kishimoto, Wataru ;

Makise, Tadahiro ;

Tawata, Shinkichi .

FOOD CHEMISTRY, 2011, 129 (03) :709-715

[6] Neobavaisoflavone stimulates osteogenesis via p38-mediated up-regulation of transcription factors and osteoid genes expression in MC3T3-E1 cells [J].

Don, Ming-Jaw ;

Lin, Lie-Chwen ;

Chiou, Wen-Fei .

PHYTOMEDICINE, 2012, 19 (06) :551-561

[7] Activation of p38 mitogen-activated protein kinase is required for osteoblast differentiation [J].

Hu, YY ;

Chan, E ;

Wang, SX ;

Li, BJ .

ENDOCRINOLOGY, 2003, 144 (05) :2068-2074

[8] Effects of growth factors and cytokines on osteoblast differentiation [J].

Hughes, FJ ;

Turner, W ;

Belibasakis, G ;

Martuscelli, G .

PERIODONTOLOGY 2000, 2006, 41 :48-72

[9] Regulation of the differentiation of osteoblasts and osteoclasts by a hot-water extract of adzuki beans (Vigna angularis) [J].

Itoh, Tomohiro ;

Nakamura, Masahiro ;

Nakamichi, Hirohisa ;

Ando, Masashi ;

Tsukamasa, Yasuyuki ;

Furuichi, Yukio .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2014, 78 (01) :92-99

[10] Phosphophoryn regulates the gene expression and differentiation of NIH3T3, MC3T3-E1, and human mesenchymal stem cells via the integrin/MAPK signaling pathway [J].

Jadlowiec, J ;

Koch, H ;

Zhang, XY ;

Campbell, PG ;

Seyedain, M ;

Sfeir, C .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (51) :53323-53330

← 1 2 3 4 →