The transcription factor bZIP68 negatively regulates cold tolerance in maize

被引：107

作者：

Li, Zhuoyang ^{[1
]}

Fu, Diyi ^{[1
]}

Wang, Xi ^{[1
]}

Zeng, Rong ^{[1
]}

Zhang, Xuan ^{[2
]}

Tian, Jinge ^{[2
]}

Zhang, Shuaisong ^{[1
]}

Yang, Xiaohong ^{[2
]}

Tian, Feng ^{[2
]}

Lai, Jinsheng ^{[2
]}

Shi, Yiting ^{[1
]}

Yang, Shuhua ^{[1
]}

机构：

[1] China Agr Univ, Coll Biol Sci, Ctr Crop Funct Genom & Mol Breeding, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China

[2] China Agr Univ, Natl Maize Improvement Ctr, State Key Lab Plant Physiol & Biochem, Ctr Crop Funct Genom & Mol Breeding, Beijing 100193, Peoples R China

来源：

PLANT CELL | 2022年 / 34卷 / 08期

基金：

中国国家自然科学基金;

关键词：

RESPONSIVE GENE-EXPRESSION; FREEZING TOLERANCE; PATHOGEN DEFENSE; STRESS TOLERANCE; CHILLING STRESS; PLANT-RESPONSES; PROTEIN-KINASES; ICE1; STABILITY; PHOSPHORYLATION; ACCLIMATION;

D O I：

10.1093/plcell/koac137

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Maize (Zea mays) originated in tropical areas and is thus susceptible to low temperatures, which pose a major threat to maize production. Our understanding of the molecular basis of cold tolerance in maize is limited. Here, we identified bZIP68, a basic leucine zipper (bZIP) transcription factor, as a negative regulator of cold tolerance in maize. Transcriptome analysis revealed that bZIP68 represses the cold-induced expression of DREB1 transcription factor genes. The stability and transcriptional activity of bZIP68 are controlled by its phosphorylation at the conserved Ser250 residue under cold stress. Furthermore, we demonstrated that the bZIP68 locus was a target of selection during early domestication. A 358-bp insertion/deletion (Indel-972) polymorphism in the bZIP68 promoter has a significant effect on the differential expression of bZIP68 between maize and its wild ancestor teosinte. This study thus uncovers an evolutionary cis-regulatory variant that could be used to improve cold tolerance in maize. The transcription factor bZIP68 negatively regulates cold tolerance in maize, and natural variation in the bZIP68 promoter underlies its difference in expression between maize and teosinte.

引用

页码：2833 / 2851

页数：19

共 64 条

[61] AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation [J].