LEIGC long non-coding RNA acts as a tumor suppressor in gastric carcinoma by inhibiting the epithelial-to-mesenchymal transition

被引：75
作者：
Han, Yuehua [1 ]
Ye, Jun [1 ]
Wu, Dang [2 ]
Wu, Pin [2 ]
Chen, Zhigang [2 ]
Chen, Jian [3 ]
Gao, Shunliang [3 ]
Huang, Jian [2 ,4 ]
机构：
[1] Zhejiang Univ, Sch Med, Dept Gastroenterol, Affiliated Hosp 2, Hangzhou 310009, Zhejiang, Peoples R China
[2] Zhejiang Univ, Sch Med, Inst Canc, Affiliated Hosp 2, Hangzhou 310009, Zhejiang, Peoples R China
[3] Zhejiang Univ, Sch Med, Dept Gen Surg, Affiliated Hosp 2, Hangzhou 310009, Zhejiang, Peoples R China
[4] Zhejiang Univ, Sch Med, Dept Oncol, Affiliated Hosp 2, Hangzhou 310009, Zhejiang, Peoples R China
来源：
BMC CANCER | 2014年 / 14卷
关键词：
Long non-coding RNA; Tumor suppressor; Gastric carcinoma; Epithelial-to-mesenchymal transition; CANCER; PROLIFERATION; SURVIVAL; EXPRESSION; DATABASE; MEG3; P53;
D O I：
10.1186/1471-2407-14-932
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background: Long non-coding RNAs have been shown to have critical regulatory roles in cancer biology. However, the contributions of lncRNAs to gastric cancer remain largely unknown. Methods: The differential expression of lncRNAs in gastric cancer and paired non-cancerous tissues were identified by microarray and validated using quantitative real-time PCR. Gastric samples from patients with gastric cancer were further analyzed for levels of a specifically downregulated lncRNA (termed as LEIGC). Results: We found that there were significantly lower levels of LEIGC expression in cancer tissue than in adjacent non-cancerous tissues in human gastric cancers (P < 0.01). Overexpression of LEIGC suppressed tumor growth and cell proliferation, and enhanced the sensitivity of gastric cancer cells to 5-fluorouracil (5-FU), whereas knockdown of LEIGC showed the opposite effect. We further demonstrated LEIGC functions by inhibiting the epithelial-tomesenchymal transition (EMT) in gastric cancer. Conclusions: Our data suggested that LEIGC is a tumor-suppressing lncRNA in gastric cancer, and led us to propose that lncRNAs may play important regulatory roles in cancer development and progression.
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页数：11
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