Identification of Sterol-Containing Domains in Vacuolar Membranes by means of Confocal Microscopy

被引：0

作者：

Nurminsky, V. N. ^{[1
]}

Nesterkina, I. S. ^{[1
]}

Spiridonova, E. V. ^{[1
]}

Rakevich, A. L. ^{[2
]}

Ozolina, N. V. ^{[1
]}

机构：

[1] Russian Acad Sci, Siberian Branch, Siberian Inst Plant Physiol & Biochem, Ul Lermontova 132, Irkutsk 664033, Russia

[2] Russian Acad Sci, Siberian Branch, Irkutsk Dept, Inst Laser Phys, Ul Lermontova 130a, Irkutsk 664033, Russia

来源：

BIOLOGICHESKIE MEMBRANY | 2017年 / 34卷 / 04期

关键词：

vacuolar membrane; lipid-protein microdomains; rafts; sterols; confocal microscopy; Filipin; LIPID RAFTS; PLASMA-MEMBRANE; CHOLESTEROL; PROBE; MICRODOMAINS; ORGANIZATION; FILIPIN; CELLS;

D O I：

10.7868/S0233475517040065

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The method of confocal microscopy and membrane probe Filipin were applied for the identification of sterol-containing domains in the tonoplast of beet root cells. The presence of sterol-containing domains in the tonoplast was confirmed using methyl-beta-cyclodextrin. It was found that sterol-containing vacuolar membrane included separate small sterol-enriched sites with a more intensive fluorescence; these sites can be attributed to raft structures. Our data demonstrate that the sterol content in isolated rafts can be assessed by confocal microscopy. A detailed analysis of the isolated rafts indicated that they contain (beta-sitosterol, stigmasterol, alpha-tocopherol, and cholesterol.

引用

页码：307 / 312

页数：6

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