Liquid chromatography determination of 10-hydroxycamptothecin in human serum by a column-switching system containing a pre-column with restricted access media and its application to a clinical pharmacokinetic study

被引：14

作者：

Ma, J ^{[1
]}

Jia, ZP ^{[1
]}

Zhang, Q ^{[1
]}

Fan, JJ ^{[1
]}

Jiang, NX ^{[1
]}

Wang, R ^{[1
]}

Xie, H ^{[1
]}

Wang, J ^{[1
]}

机构：

[1] Gen Hosp Lanzhou Command PLA, Dept Pharm, Lanzhou 730050, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 796卷 / 01期

关键词：

pharmacokinetics; restricted access media; 10-hydroxycamptothecin;

D O I：

10.1016/S1570-0232(03)00612-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, rapid, sensitive column-switching HPLC method is described for the analysis of the 10-hydroxycamptothecin (HCPT) in human serum. A pre-column containing restricted access media (RAM) is used for the sample clean-up and trace enrichment and is combined with a C-18 column for the final separation. The analytical time is 8 min. The HCPT is monitored with fluorescence detector, excitation and emission wavelengths being 385 and 539 nm, respectively. There is a linear response range of 1-1000 ng/ml with correlation coefficient of 0.998 while the limit of quantification is 0.1 ng/ml. The intra-day and inter-day variations are less than 5%. This analytic procedure has been applied to a pharmacokinetic study of HCPT in clinical patients and the pharmacokinetic parameters of one-compartment model are calculated. (C) 2003 Elsevier B.V All rights reserved.

引用

页码：195 / 200

页数：6

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