The Translational Potential of Electrochemical DNA-Based Liquid Biopsy

被引：24
作者：
Miranda-Castro, Rebeca [1 ,2 ]
Palchetti, Ilaria [3 ]
de-los-Santos-Alvarez, Noemi [1 ,2 ]
机构：
[1] Univ Oviedo, Dept Quim Fis & Analit, Oviedo, Spain
[2] Inst Invest Sanitaria Principado Asturias, Oviedo, Spain
[3] Univ Firenze, Dipartimento Chim Ugo Schiff, Florence, Italy
来源：
FRONTIERS IN CHEMISTRY | 2020年 / 8卷
关键词：
cancer; liquid biopsy; nucleic acids; electrochemical biosensors; ctDNA; exosomes; CTCs; aptamer; LABEL-FREE; CARCINOEMBRYONIC ANTIGEN; AMPLIFICATION; APTASENSOR; BIOSENSOR; BIOMARKERS; PROTEIN; PLASMA; SENSOR; GENE;
D O I：
10.3389/fchem.2020.00143
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Latest technological advancement has tremendously expanded the knowledge on the composition of body fluids and the cancer-associated changes, which has fueled the replacement of invasive biopsies with liquid biopsies by using appropriate specific receptors. DNA emerges as a versatile analytical reagent in electrochemical devices for hybridization-based or aptamer-based recognition of all kind of biomarkers. In this mini review, we briefly introduce the current affordable targets (tumor-derived nucleic acids, circulating tumor cells and exosomes) in body fluids, and then we provide an overview of selected electrochemical methods already applied in clinical samples by dividing them into three large categories according to sample type: red (blood), yellow (urine), and white (saliva and sweat) diagnostics. This review focuses on the hurdles of the complex matrices rather than a comprehensive and detailed revision of the format schemes of DNA-based electrochemical sensing. This diverse perspective compiles some challenges that are often forgotten and critically underlines real sample analysis or clinical validation assays. Finally, the needs and trends to reach the market are briefly outlined.
引用
收藏
页数：10
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