Biological control on gastrointestinal nematodes in cattle with association of nematophagous fungi

被引：9
作者：
Fernandes, Fernanda Mara [1 ]
Aguiar, Anderson Rocha [2 ]
Costa Silva, Laryssa Pinheiro [2 ]
Senna, Thiago [2 ]
Kramer de Mello, Ingrid Ney [1 ]
de Oliveira, Thais [1 ]
Freitas, Samuel Galvao [1 ]
Silveira, Wendeo Ferreira [1 ]
Braga, Fabio Ribeiro [2 ]
Araujo, Jackson Victor [1 ]
机构：
[1] Univ Fed Vicosa, Dept Vet Med, Vicosa, MG, Brazil
[2] Univ Vila Velha ES, Dept Parasitol, Vila Velha, ES, Brazil
来源：
BIOCONTROL SCIENCE AND TECHNOLOGY | 2017年 / 27卷 / 12期
关键词：
Biological control; ecofriendly; biotechnology; use of natural enemies; nematophagous fungi; DUDDINGTONIA-FLAGRANS; MONACROSPORIUM-THAUMASIUM; PELLET FORMULATION; INFECTIVE LARVAE; SODIUM ALGINATE; BRAZIL; CALVES;
D O I：
10.1080/09583157.2017.1406063
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
This study aimed to evaluate the efficacy of the association of the nematophagous fungi (Duddingtonia flagrans-AC001); (Pochonia chlamydosporia-VC4) and (Arthrobotrys robusta-I31) in a pelletised formulation of a sodium alginate matrix. The viability and activity of pellet germination and fungal activity (after encapsulation) were evaluated using in vitro and in vivo tests. Next, 12 heads of cattle, Dutch mestizo x zebu, with an average age of 12 months were dewormed with an anthelmintic. Next, 20 days after treatment with the anthelmintic, the animals were randomly divided into two groups of six animals each, and placed in two paddocks with 7.0 ha each of Brachiaria decumbens with historical grazing by animals naturally infected by gastrointestinal nematode parasites. At first, each animal was treated with 2 g of pellets per 10 kg of animal, containing the associated fungi (AC001 + VC4 + I31) administered twice a week in conjunction with commercial feed. Each animal in the control group received 2 g of pellets without mycelia added to the feed. The percentage reduction of infective larvae in the in vitro test was 94% (p < .01). In the in vivo test, the treated animals with fungal association had lower egg counts per gram of faeces (p < .01) compared to the control group animals - a reduction of 91.8%. The reduction in the number of infective larvae recovered from the pasture was 27.5% at a distance of 0-20 cm from the stool bulk, and 26.7% at a distance of 20-40 cm. This association (AC001 + VC4 + I31) of nematophagous fungi was effective in nematode control.
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页码：1445 / 1453
页数：9
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