The use of the LightCycler for the detection of Y chromosome SNPs

被引：21

作者：

Lareu, M ^{[1
]}

Puente, J ^{[1
]}

Sobrino, B ^{[1
]}

Quintáns, B ^{[1
]}

Brión, M ^{[1
]}

Carracedo, A ^{[1
]}

机构：

[1] Univ Santiago de Compostela, Inst Legal Med, E-15705 Santiago De Compostela, Galicia, Spain

来源：

FORENSIC SCIENCE INTERNATIONAL | 2001年 / 118卷 / 2-3期

关键词：

SNPs; Y chromosome; LightCycler; mix stains;

D O I：

10.1016/S0379-0738(01)00386-3

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

A novel methodology based on PCR monitoring on-line with fluorescent formats using the LightCycler for Y chromosome SNP typing is proposed. The main advantages of the system are the time necessary for the analysis (which is around 20 min), the robustness and the accuracy of the method and especially its sensitivity, which permits the detection of the male component in male-female mixtures up to 1:300 for some of the SNPs. Singleplexes of four different SNPs (M9, sY81, SRY-1532 and SRY-2627) as well as two duplexes (M9 and sY81 on the one hand and SRY-1532 and SRY-2627 on the other) were efficiently implemented. A simultaneous amplification and analysis of the four SNPs is also possible. It seems difficult with the current methodology to implement more than a quadruplex. (C) 2001 Elsevier Science ireland Ltd. All rights reserved.

引用

页码：163 / 168

页数：6

共 15 条

[1] ELECTROPHORETIC HUMAN-LEUKOCYTE ANTIGEN HLA-DQA1 DNA TYPING AFTER POLYMERASE CHAIN-REACTION AMPLIFICATION [J].

BARROS, F ;

CARRACEDO, A ;

LAREU, MV ;

RODRIGUEZCALVO, MS .

ELECTROPHORESIS, 1991, 12 (12) :1041-1045

[2] DETECTION OF POLYMORPHISMS OF HUMAN DNA AFTER POLYMERASE CHAIN-REACTION BY MINIATURIZED SDS-PAGE [J].

BARROS, F ;

LAREU, MV ;

CARRACEDO, A .

FORENSIC SCIENCE INTERNATIONAL, 1992, 55 (01) :27-36

[3] Integrated amplification and detection of the C677T point mutation in the methylenetetrahydrofolate reductase gene by fluorescence resonance energy transfer and probe melting curves [J].

Bernard, PS ;

Lay, MJ ;

Wittwer, CT .

ANALYTICAL BIOCHEMISTRY, 1998, 255 (01) :101-107

[4]

Brión M, 2000, INT CONGR SER, P263

[5] Progress in methodology and standards in European molecular genetics laboratories [J].

Carracedo, A ;

Barros, F ;

Loidi, L ;

Dominguez, F .

CLINICA CHIMICA ACTA, 1998, 278 (02) :163-169

[6] Alternative primers for DYS391 typing:: advantages of their application to forensic genetics [J].

Gusmao, L ;

González-Neira, A ;

Sánchez-Diz, P ;

Lareu, MV ;

Amorim, A ;

Carracedo, A .

FORENSIC SCIENCE INTERNATIONAL, 2000, 112 (01) :49-57

[7] Recent male-mediated gene flow over a linguistic barrier in Iberia, suggested by analysis of a Y-chromosomal DNA polymorphism [J].

Hurles, ME ;

Veitia, R ;

Arroyo, E ;

Armenteros, M ;

Bertranpetit, J ;

Pérez-Lezaun, A ;

Bosch, E ;

Shlumukova, M ;

Cambon-Thomsen, A ;

McElreavey, K ;

de Munain, AL ;

Röhl, A ;

Wilson, IJ ;

Singh, LJ ;

Pandya, A ;

Santos, FR ;

Tyler-Smith, C ;

Jobling, MA .

AMERICAN JOURNAL OF HUMAN GENETICS, 1999, 65 (05) :1437-1448

[8] European y-chromosomal lineages in Polynesians: A contrast to the population structure revealed by mtDNA [J].

Hurles, ME ;

Irven, C ;

Nicholson, J ;

Taylor, PG ;

Santos, FR ;

Loughlin, J ;

Jobling, MA ;

Sykes, BC .

AMERICAN JOURNAL OF HUMAN GENETICS, 1998, 63 (06) :1793-1806

[9] Multiplexing of Y chromosome specific STRs and performance for mixed samples [J].

Prinz, M ;

Boll, K ;

Baum, H ;

Shaler, B .

FORENSIC SCIENCE INTERNATIONAL, 1997, 85 (03) :209-218

[10] A new method for the evaluation of matches in non-recombining genomes:: application to Y-chromosomal short tandem repeat (STR) haplotypes in European males [J].

Roewer, L ;

Kayser, M ;

de Knijff, P ;

Anslinger, K ;

Betz, A ;

Caglià, A ;

Corach, D ;

Füredi, S ;

Henke, L ;

Hidding, M ;

Kärgel, HJ ;

Lessig, R ;

Nagy, M ;

Pascali, VL ;

Parson, W ;

Rolf, B ;

Schmitt, C ;

Szibor, R ;

Teifel-Greding, J ;

Krawczak, M .

FORENSIC SCIENCE INTERNATIONAL, 2000, 114 (01) :31-43

← 1 2 →