IN VITRO AND IN VIVO ASSAYS OF PROTEIN KINASE CK2 ACTIVITY

被引：0

作者：

Prudent, Renaud ^{[1
]}

Sautel, Celine F. ^{[1
]}

Moucadel, Virginie ^{[1
]}

Laudet, Beatrice ^{[1
]}

Filhol, Odile ^{[1
]}

Cochet, Claude ^{[1
]}

机构：

[1] Univ Grenoble 1, INSERM, U873, CEA,IRTSV LTS, Grenoble, France

来源：

METHODS IN ENZYMOLOGY, VOLUME 485: CONSTITUTIVE ACTIVITY IN RECEPTORS AND OTHER PROTEINS, PART B | 2010年 / 485卷

关键词：

REGULATORY SUBUNIT; INHIBITION; CELLS;

D O I：

10.1016/S0076-6879(10)85031-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein kinase CK2 (formerly casein kinase 2) is recognized as a central component in the control of the cellular homeostasis; however, much remains unknown regarding its regulation and its implication in cellular transformation and carcinogenesis. Moreover, study of CK2 function and regulation in a cellular context is complicated by the dynamic multisubunit architecture of this protein kinase. Although a number of robust techniques are available to assay CK2 activity in vitro, there is a demand for sensitive and specific assays to evaluate its activity in living cells. We hereby provide a detailed description of several assays for monitoring the CK2 activity and its subunit interaction in living cells. The guidelines presented herein should enable researchers in the field to establish strategies for cellular screenings of CK2 inhibitors.

引用

页码：597 / 610

页数：14

共 50 条

[31] The role of protein kinase CK2 in the regulation of the insulin production of pancreatic islets
Meng, Rui
Goetz, Claudia
Montenarh, Mathias
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2010, 401 (02) : 203 - 206
[32] Protein Kinase CK2 Content in GL261 Mouse Glioblastoma
Ferrer-Font, Laura
Alcaraz, Estefania
Plana, Maria
Paula Candiota, Ana
Itarte, Emilio
Arus, Carles
PATHOLOGY & ONCOLOGY RESEARCH, 2016, 22 (03) : 633 - 637
[33] Adiponectin receptor 1 interacts with both subunits of protein kinase CK2
Juhl, Cathleen
Moerl, Karin
Beck-Sickinger, Annette G.
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2011, 356 (1-2) : 185 - 189
[34] Addiction to protein kinase CK2: A common denominator of diverse cancer cells?
Ruzzene, Maria
Pinna, Lorenzo A.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2010, 1804 (03): : 499 - 504
[35] Biochemical and cellular mechanism of protein kinase CK2 inhibition by deceptive curcumin
Cozza, Giorgio
Zonta, Francesca
Dalle Vedove, Andrea
Venerando, Andrea
Dall'Acqua, Stefano
Battistutta, Roberto
Ruzzene, Maria
Lolli, Graziano
FEBS JOURNAL, 2020, 287 (09) : 1850 - 1864
[36] Crystal structures of catalytic and regulatory subunits of rat protein kinase CK2
Zhou WeiHong
Qin XiaoHong
Yan XiaoJie
Xie XingQiao
Li Liang
Fang ShaSha
Long JiaFu
Adelman, John
Tang Wei-Jen
Shen YueQuan
CHINESE SCIENCE BULLETIN, 2009, 54 (02): : 220 - 226
[37] Role of polyamines in determining the cellular response to chemotherapeutic agents: modulation of protein kinase CK2 expression and activity
Kreutzer, Jan N.
Olsen, Birgitte B.
Lech, Karolina
Issinger, Olaf-Georg
Guerra, Barbara
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2011, 356 (1-2) : 149 - 158
[38] Efficient development of stable and highly functionalised peptides targeting the CK2α/CK2β protein-protein interaction
Iegre, Jessica
Brear, Paul
Baker, David J.
Tan, Yaw Sing
Atkinson, Eleanor L.
Sore, Hannah F.
O' Donovan, Daniel H.
Verma, Chandra S.
Hyvoenen, Marko
Spring, David R.
CHEMICAL SCIENCE, 2019, 10 (19) : 5056 - 5063
[39] Functional Analysis of Protein Kinase CK2 of the Human Malaria Parasite Plasmodium falciparum
Holland, Zoe
Prudent, Renaud
Reiser, Jean-Baptiste
Cochet, Claude
Doerig, Christian
EUKARYOTIC CELL, 2009, 8 (03) : 388 - 397
[40] Protein kinase CK2 is a critical regulator of epithelial homeostasis in chronic intestinal inflammation
Koch, S.
Capaldo, C. T.
Hilgarth, R. S.
Fournier, B.
Parkos, C. A.
Nusrat, A.
MUCOSAL IMMUNOLOGY, 2013, 6 (01) : 136 - 145

← 1 2 3 4 5 →