IN VITRO AND IN VIVO ASSAYS OF PROTEIN KINASE CK2 ACTIVITY

被引：0
作者：
Prudent, Renaud [1 ]
Sautel, Celine F. [1 ]
Moucadel, Virginie [1 ]
Laudet, Beatrice [1 ]
Filhol, Odile [1 ]
Cochet, Claude [1 ]
机构：
[1] Univ Grenoble 1, INSERM, U873, CEA,IRTSV LTS, Grenoble, France
来源：
METHODS IN ENZYMOLOGY, VOLUME 485: CONSTITUTIVE ACTIVITY IN RECEPTORS AND OTHER PROTEINS, PART B | 2010年 / 485卷
关键词：
REGULATORY SUBUNIT; INHIBITION; CELLS;
D O I：
10.1016/S0076-6879(10)85031-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Protein kinase CK2 (formerly casein kinase 2) is recognized as a central component in the control of the cellular homeostasis; however, much remains unknown regarding its regulation and its implication in cellular transformation and carcinogenesis. Moreover, study of CK2 function and regulation in a cellular context is complicated by the dynamic multisubunit architecture of this protein kinase. Although a number of robust techniques are available to assay CK2 activity in vitro, there is a demand for sensitive and specific assays to evaluate its activity in living cells. We hereby provide a detailed description of several assays for monitoring the CK2 activity and its subunit interaction in living cells. The guidelines presented herein should enable researchers in the field to establish strategies for cellular screenings of CK2 inhibitors.
引用
收藏
页码：597 / 610
页数：14
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