The Ras related GTPase Miro is not required for mitochondrial transport in Dictyostelium discoideum

被引:35
作者
Vlahou, Georgia [3 ]
Elias, Marek [4 ,5 ,6 ]
von Kleist-Retzow, Juergen-Christoph [7 ,8 ]
Wiesner, Rudolf J. [8 ,9 ,10 ]
River, Francisco [1 ,2 ,3 ]
机构
[1] Univ Hull, Hull York Med Sch, Ctr Biomed Res, Kingston Upon Hull HU6 7RX, N Humberside, England
[2] Univ Hull, Dept Biol Sci, Kingston Upon Hull HU6 7RX, N Humberside, England
[3] Univ Cologne, Fak Med, Zentrum Biochem, D-50931 Cologne, Germany
[4] Charles Univ Prague, Fac Sci, Dept Bot, Prague 12801 2, Czech Republic
[5] Charles Univ Prague, Fac Sci, Dept Parasitol, Prague 12801 2, Czech Republic
[6] Univ Ostrava, Fac Sci, Dept Biol & Ecol, Slezska Ostrava 71000, Czech Republic
[7] Univ Cologne, Fak Med, Klin & Poliklin Kinderheilkunde, D-50937 Cologne, Germany
[8] Univ Cologne, CMMC, D-50931 Cologne, Germany
[9] Univ Cologne, Fak Med, Inst Vegetat Physiol, D-50931 Cologne, Germany
[10] Univ Cologne, Inst Genet, CECAD Cologne Excellence Cluster Cellular Stress, D-50674 Cologne, Germany
关键词
Miro; Mitochondrial transport; Small GTPase; Dictyostelium; ATYPICAL RHO-GTPASES; AXONAL-TRANSPORT; PROTEIN; DYNAMICS; IDENTIFICATION; PURIFICATION; MORPHOLOGY; ALGORITHM; MOTILITY; MUTANTS;
D O I
10.1016/j.ejcb.2010.10.012
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Ras-related GTPases of the Miro family have been implicated in mitochondrial homeostasis and microtubule-dependent transport. They consist of two GTP-binding domains separated by calcium-binding motifs and of a C-terminal transmembrane domain that targets the protein to the outer mitochondrial membrane. We disrupted the single Miro-encoding gene in Dictyostelium discoideum and observed a substantial growth defect that we attribute to a decreased mitochondrial mass and cellular ATP content. However, mutant cells even showed an increased rate of oxygen consumption, while glucose consumption, mitochondrial transmembrane potential and production of reactive oxygen species were unaltered. Processes characteristic of the multicellular stage of the D. discoideum life cycle were also unaltered. Although mitochondria occasionally use microtubules for transport in D. discoideum, their size and distribution were not visibly affected. We found Miro in all branches of the eukaryotic tree with the exception of a few protist lineages (mainly those lacking typical mitochondria). Trypanosomatids and ciliates possess structurally unique homologs lacking the N-terminal or the C-terminal GTPase domain, respectively. We propose that in D. discoideum, as in yeasts and plants, Miro plays roles in mitochondrial homeostasis, but the ability to build a complex that regulates its association to kinesin for microtubule-dependent transport probably arose in metazoans. (C) 2010 Elsevier GmbH. All rights reserved.
引用
收藏
页码:342 / 355
页数:14
相关论文
共 58 条
  • [1] ACTIVATION OF THE NADPH OXIDASE INVOLVES THE SMALL GTP-BINDING PROTEIN P21RAC1
    ABO, A
    PICK, E
    HALL, A
    TOTTY, N
    TEAHAN, CG
    SEGAL, AW
    [J]. NATURE, 1991, 353 (6345) : 668 - 670
  • [2] ISOLATION OF DICTYOSTELIUM-DISCOIDEUM CYTOKINESIS MUTANTS BY RESTRICTION ENZYME-MEDIATED INTEGRATION OF THE BLASTICIDIN-S RESISTANCE MARKER
    ADACHI, H
    HASEBE, T
    YOSHINAGA, K
    OHTA, T
    SUTOH, K
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 205 (03) : 1808 - 1814
  • [3] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    Altschul, SF
    Madden, TL
    Schaffer, AA
    Zhang, JH
    Zhang, Z
    Miller, W
    Lipman, DJ
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
  • [4] Ausubel FM., 2001, CURRENT PROTOCOLS MO
  • [5] CARBOHYDRATE AND OTHER EPITOPES OF THE CONTACT SITE-A GLYCOPROTEIN OF DICTYOSTELIUM-DISCOIDEUM AS CHARACTERIZED BY MONOCLONAL-ANTIBODIES
    BERTHOLDT, G
    STADLER, J
    BOZZARO, S
    FICHTNER, B
    GERISCH, G
    [J]. CELL DIFFERENTIATION, 1985, 16 (03): : 187 - 202
  • [6] Diverse cytopathologies in mitochondrial disease are caused by AMP-activated protein kinase signaling
    Bokko, Paul B.
    Francione, Lisa
    Bandala-Sanchez, Esther
    Ahmed, Afsar U.
    Annesley, Sarah J.
    Huang, Xiuli
    Khurana, Taruna
    Kimmel, Alan R.
    Fisher, Paul R.
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2007, 18 (05) : 1874 - 1886
  • [7] Borgese N, 2003, BIOCHEM SOC T, V31, P1238
  • [8] Kingdoms Protozoa and Chromista and the eozoan root of the eukaryotic tree
    Cavalier-Smith, Thomas
    [J]. BIOLOGY LETTERS, 2010, 6 (03) : 342 - 345
  • [9] A developmentally regulated kinesin-related motor protein from Dictyostelium discoideum
    de Hostos, EL
    McCaffrey, G
    Sucgang, R
    Pierce, DW
    Vale, RD
    [J]. MOLECULAR BIOLOGY OF THE CELL, 1998, 9 (08) : 2093 - 2106
  • [10] Subsecond reorganization of the actin network in cell motility and chemotaxis
    Diez, S
    Gerisch, G
    Anderson, K
    Müller-Taubenberger, A
    Bretschneider, T
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (21) : 7601 - 7606