Detection of Chlamydophila psittaci by Using SYBR Green Real-Time PCR

被引：7
|
作者：
Okuda, Hideko [2 ]
Ohya, Kenji [1 ,2 ]
Shiota, Yukihiro [2 ]
Kato, Hiroshi [2 ]
Fukushi, Hideto [1 ,2 ]
机构：
[1] Gifu Univ, Fac Appl Biol Sci, Lab Vet Microbiol, Gifu 5011193, Japan
[2] Gifu Univ, United Grad Sch Vet Sci, Dept Appl Vet Sci, Gifu 5011193, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2011年 / 73卷 / 02期
基金：
日本学术振兴会;
关键词：
avian chlamydiosis; Chlamydophila psittaci; real-time PCR; SYBR Green dye; MAMMALIAN CHLAMYDIA-PSITTACI; OUTER-MEMBRANE PROTEIN; GENETIC DIVERSITY; BIRDS; INFECTIONS; DIAGNOSIS; ANTIGEN; ASSAY;
D O I：
10.1292/jvms.10-0222
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Chlamydophila psittaci is the causative agent of human psittacosis and avian chlamydiosis. This zoonotic pathogen is frequently transmitted from infected birds to humans. Therefore proper and rapid detection of C. psittaci in birds is important to control this disease. We developed a method for detecting C. psittaci by using SYBR Green Real-time PCR based on targeting the cysteine-rich protein gene (envB) of C. psittaci. This one step procedure was highly sensitive and rapid for detection and quantification of C. psittaci from fecal samples. This assay was also able to detect other zoonotic Chlamydophila species such as C. abortus and C. fells. The assay is well suited for use as a routine detection method in veterinary medicine.
引用
收藏
页码：249 / 254
页数：6
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