Optogenetic control of calcium influx in mammalian cells

被引：2

作者：

Lee, Yi-Tsang ^{[1
]}

Chen, Rui ^{[1
]}

Zhou, Yubin ^{[1
]}

He, Lian ^{[1
]}

机构：

[1] Texas A&M Univ, Inst Biosci & Technol, Ctr Translat Canc Res, Houston, TX 77843 USA

来源：

ION CHANNELS: CHANNEL CHEMICAL BIOLOGY, ENGINEERING, AND PHYSIOLOGICAL FUNCTION | 2021年 / 654卷

关键词：

STIM1; PROTEINS; BINDING; DOMAIN; LIGHT; ORAI;

D O I：

10.1016/bs.mie.2021.02.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Optogenetics combines optics and genetics to enable non-invasive interrogation of cell physiology at an unprecedented high spatiotemporal resolution. Here, we introduce Opto-CRAC as a set of genetically-encoded calcium actuators (GECAs) engineered from the calcium release-activated calcium (CRAC) channel, which has been tailored for optical control of calcium entry and calcium-dependent physiological responses in nonexcitable cells and tissues. We describe a detailed protocol for applying Opto-CRAC as an optogenetic tool to achieve photo-tunable control over intracellular calcium signals and calcium-dependent gene expression in mammalian cells.

引用

页码：255 / 270

页数：16

共 33 条

[1] Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics [J].

Akerboom, Jasper ;

Calderon, Nicole Carreras ;

Tian, Lin ;

Wabnig, Sebastian ;

Prigge, Matthias ;

Tolo, Johan ;

Gordus, Andrew ;

Orger, Michael B. ;

Severi, Kristen E. ;

Macklin, John J. ;

Patel, Ronak ;

Pulver, Stefan R. ;

Wardill, Trevor J. ;

Fischer, Elisabeth ;

Schueler, Christina ;

Chen, Tsai-Wen ;

Sarkisyan, Karen S. ;

Marvin, Jonathan S. ;

Bargmann, Cornelia I. ;

Kim, Douglas S. ;

Kugler, Sebastian ;

Lagnado, Leon ;

Hegemann, Peter ;

Gottschalk, Alexander ;

Schreiter, Eric R. ;

Looger, Loren L. .

FRONTIERS IN MOLECULAR NEUROSCIENCE, 2013, 6

[2] Optogenetic protein clustering and signaling activation in mammalian cells [J].

Bugaj, Lukasz J. ;

Choksi, Atri T. ;

Mesuda, Colin K. ;

Kane, Ravi S. ;

Schaffer, David V. .

NATURE METHODS, 2013, 10 (03) :249-252

[3] Ultrasensitive fluorescent proteins for imaging neuronal activity [J].

Chen, Tsai-Wen ;

Wardill, Trevor J. ;

Sun, Yi ;

Pulver, Stefan R. ;

Renninger, Sabine L. ;

Baohan, Amy ;

Schreiter, Eric R. ;

Kerr, Rex A. ;

Orger, Michael B. ;

Jayaraman, Vivek ;

Looger, Loren L. ;

Svoboda, Karel ;

Kim, Douglas S. .

NATURE, 2013, 499 (7458) :295-+

[4] Structure of a flavin-binding plant photoreceptor domain: Insights into light-mediated signal transduction [J].

Crosson, S ;

Moffat, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (06) :2995-3000

[5] High-performance calcium sensors for imaging activity in neuronal populations and microcompartments [J].

Dana, Hod ;

Sun, Yi ;

Mohar, Boaz ;

Hulse, Brad K. ;

Kerlin, Aaron M. ;

Hasseman, Jeremy P. ;

Tsegaye, Getahun ;

Tsang, Arthur ;

Wong, Allan ;

Patel, Ronak ;

Macklin, John J. ;

Chen, Yang ;

Konnerth, Arthur ;

Jayaraman, Vivek ;

Looger, Loren L. ;

Schreiter, Eric R. ;

Svoboda, Karel ;

Kim, Douglas S. .

NATURE METHODS, 2019, 16 (07) :649-+

[6] A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function [J].

Feske, S ;

Gwack, Y ;

Prakriya, M ;

Srikanth, S ;

Puppel, SH ;

Tanasa, B ;

Hogan, PG ;

Lewis, RS ;

Daly, M ;

Rao, A .

NATURE, 2006, 441 (7090) :179-185

[7]

GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440

[8] Engineering of a bona fide light-operated calcium channel [J].

He, Lian ;

Wang, Liuqing ;

Zeng, Hongxiang ;

Tan, Peng ;

Ma, Guolin ;

Zheng, Sisi ;

Li, Yaxin ;

Sun, Lin ;

Dou, Fei ;

Siwko, Stefan ;

Huang, Yun ;

Wang, Youjun ;

Zhou, Yubin .

NATURE COMMUNICATIONS, 2021, 12 (01)

[9] Optical control of membrane tethering and interorganellar communication at nanoscales [J].

He, Lian ;

Jing, Ji ;

Zhu, Lei ;

Tan, Peng ;

Ma, Guolin ;

Zhang, Qian ;

Nhung T. Nguyen ;

Wang, Junfeng ;

Zhou, Yubin ;

Huang, Yun .

CHEMICAL SCIENCE, 2017, 8 (08) :5275-5281

[10] Near-infrared photoactivatable control of Ca2+ signaling and optogenetic immunomodulation [J].

He, Lian ;

Zhang, Yuanwei ;

Ma, Guolin ;

Tan, Peng ;

Li, Zhanjun ;

Zang, Shengbing ;

Wu, Xiang ;

Jing, Ji ;

Fang, Shaohai ;

Zhou, Lijuan ;

Wang, Youjun ;

Huang, Yun ;

Hogan, Patrick G. ;

Han, Gang ;

Zhou, Yubin .

ELIFE, 2015, 4

← 1 2 3 4 →