Birth of puppies of predetermined sex after artificial insemination with a low number of sex-sorted, frozen-thawed spermatozoa in field conditions

被引：3

作者：

Wei, Yun-Fang ^{[1
]}

Chen, Fang-Liang ^{[1
]}

Tang, Shu-Sheng ^{[1
]}

Mao, Ai-Guo ^{[1
]}

Li, Li-Guang ^{[1
]}

Cheng, Lu-Guang ^{[1
]}

Chen, Chao ^{[1
]}

Li, Fei-Xiang ^{[1
]}

Wang, Bin ^{[1
]}

Xu, Tao ^{[1
]}

Zhang, Yue-Jun ^{[1
]}

Li, Jing ^{[1
]}

Wan, Jiu-Sheng ^{[1
]}

机构：

[1] Minist Publ Secur, Kunming Police Dog Base, Kunming 650204, Yunnan, Peoples R China

来源：

ANIMAL SCIENCE JOURNAL | 2017年 / 88卷 / 08期

关键词：

dog; flow cytometry; insemination; pregnancy; sex ratio; sperm; OF-THE-ART; MAMMALIAN SPERM; INTRAUTERINE INSEMINATION; INTRATUBAL INSEMINATION; HOECHST; 33342; CANINE SEMEN; FLOW; DOGS; CRYOPRESERVATION; PRESELECTION;

D O I：

10.1111/asj.12763

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The aim of this study was to evaluate fertility and sex ratios after artificial insemination in dogs under field conditions. Semen was cryopreserved as unsorted (control) or was separated into X-and Y-chromosome-bearing sperm using a cell sorter. Sixty female dogs were inseminated with frozen-thawed spermatozoa of 100 x 10(6) unsorted (a dose in practice) and 4 x 10(6) sorted (X and Y group, respectively). A total of 20 dogs became pregnant and 126 puppies were born from the three groups. The percentage of parturition was similar for the X (5/20; 25.0%) and Y (4/20; 20.0%) group (P > 0.05), but lower than controls (11/20; 55.0%) (P < 0.05). Ultimately 28 out of the 32 puppies produced from X group were female (87.5%) and 19/22 (86.4%) puppies of Y group were male. In contrast, sex ratio (51.4% to 48.6%) in the control was significantly different from the X, Y group (P < 0.05). However, male and female puppies in the control had similar birth weights and weaning weights to those from the X and Y groups. This preliminary information indicated that normal puppies of predicted sex can be produced with low numbers of sorted cryopreserved dog spermatozoa at a farm level, making sperm-sexing technology potentially applicable for elite breeding units.

引用

页码：1232 / 1238

页数：7

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