A bright two-photon fluorescent probe for real-time monitoring autophagy in living cells

被引：17

作者：

Jiang, Chuankun ^{[1
,2
]}

Li, Longchun ^{[3
]}

Jiang, Jiacheng ^{[1
,2
]}

Hou, Lilin ^{[1
,2
]}

Fang, Gemin ^{[1
,2
]}

Yu, Haizhu ^{[1
,2
]}

Meng, Xiangming ^{[1
,2
,3
]}

机构：

[1] Anhui Univ, Sch Chem & Chem Engn, Anhui Prov Key Lab Chem Inorgan Organ Hybrid Func, Hefei 230601, Peoples R China

[2] Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Peoples R China

[3] Anhui Agr Univ, Sch Sci, Dept Appl Chem, Hefei 230036, Peoples R China

来源：

CHINESE CHEMICAL LETTERS | 2020年 / 31卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Two-photon; Lysosome-targeting; Polarity; Real-time; Autophagy;

D O I：

10.1016/j.cclet.2019.07.046

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel donor-acceptor (D-A) type of two-photon (TP) fluorescent probe, i.e. Lyso-OSC, based on the lysosome-targeting morpholine group was developed. The polarity sensing coumarin group was functionalized as the acceptor and the 1-vinyl-4-methoxybenzene group was engineered as the donor. The fluorescence intensity and emission maximum wavelength of Lyso-OSC are highly sensitive to the polarity changes of solvent. The two-photon absorption cross-section and tissue penetration depth are up to 254 GM and 150 mu m, respectively. The strong fluorescence, high sensitivity to polarity, low cytotoxicity, and accurate lysosome-targeting ability entail Lyso-OSC the excellent performance in detecting the polarity changes of cellular environment. To this end, a bright, real-time imaging autophagy of living cells has been achieved. (C) 2019 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.

引用

页码：447 / 450

页数：4

共 30 条

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